农杆菌介导的ARGOS-LIKE转基因苋菜上胚轴的转化

Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020 Pub Date : 2020-12-30 DOI:10.28983/plamic2020.244

R. Taipova, B. R. Kuluev

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引用次数: 0

摘要

本文报道了农杆菌介导拟南芥(Arabidopsis thaliana) 35S启动子控制的ARGOS-LIKE转基因对苋菜(Amaranthus cruentus)品种“Bagryanyi”上胚轴片段的转化研究结果。农杆菌介导的上胚轴茎段再生采用含有13 μM 6-苄基氨基嘌呤和1 μM α-萘乙酸的MS培养基。在MS培养基中加入10 mg/L的潮霉素B进行转基因芽的筛选。

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Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene

The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.

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Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020

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