{"title":"米沙汗角鲨烯单脂肪酸甾酯达里大豆油脱臭馏出物","authors":"Setiyo Gunawan, Yi Hsu Ju","doi":"10.5614/jtki.2008.7.2.3","DOIUrl":null,"url":null,"abstract":"Depending on conditions in the refining process, soybean oil deodorizer distillate (SODD) contains 42-51% free fatty acids (FFA) and 16-25% triacylglycerol (TAG). Bioactive compounds such as tocopherols, free phytosterols, fatty acid steryl esters (FASE) and squalene also make up a significant portion of SODD. The efficient removal of FFA and TAG is a crucial step in the isolation and purification of bioactive compounds from SODD. In this work, a modified soxhlet extraction technique was developed and its optimal operation conditions were determined for the efficient removal of FFA and TAG from SODD. Starting with SODD that contains 3.53% FASE and 1.99% squalene, it was possible to obtain a fraction enriched with FASE (22.0%, recovery 91.3%) and squalene (8.63%, recovery 100%) by this modified soxhlet extraction under the following operation conditions: SODD/(silica gel) = 1/3 (w/w), extraction temperature = -6°C, number of extraction/h = 8.7. FFA remained in this FASE and squalene enriched fraction is 38.7% and there was no detectable TAG. The percentage removal of FFA and TAG for this FASE and squalene enriched fraction are 84.29% and 100%, respectively. The advantages of modified soxhlet extraction over open-column chromatography are that less solvent usage, larger sample size per batch and shorter operation time.Keywords: Fatty acid steryl ester; free fatty acid; modified soxhlet extraction; soybean oil deodorizer distillate; squalene; triacylglycerol AbstrakDalam proses pemurnian minyak kedelai, produk samping dari proses penghilangan bau (soybean oil deodorizer distillate, SODD) mengandung 42-52% free fatty acids (FFA) dan 18-28% triacylglycerols (TAG). Komponen bioaktif seperti tocopherols, free phytosterols, fatty acid steryl esters (FASE) dan squalene juga mempunyai kontribusi yang besar dalam komposisi SODD. Efisiensi penghilangan FFA dan TAG adalah langkah yang sangat penting dalam pemisahan dan pemurnian komponen bioaktif dari SODD. Dalam penelitian ini, modifikasi soxhlet ekstraksi telah ditemukan dan optimisasi kondisi operasi ditentukan berdasarkan efisiensi penghilangan FFA dan TAG dari SODD. Fraksi lemak non polar (nonpolar lipid fraction, NPLF) dengan kandungan FASE (22.0%, recovery 91.3%) dan squalene (8.63%, recovery 100%) telah diperoleh dengan menggunakan modifikasi soxhlet ektraksi dengan kondisi operasi: SODD/(silica gel) = 1/3 (w/w), dan suhu ekstraksi = -6°C. FFA dan TAG yang tersisa di NPLF secara berurutan adalah 38.7% dan 0%. Persentase penghilangan FFA dan TAG di NPLF secara berurutan adalah 84.29% and 100%. Keuntungan menggunakan modifikasi soxhlet ekstraksi dibandingkan dengan silica gel column chromatography adalah sedikitnya pelarut yang digunakan, besarnya sampel per unit batch yang digunakan, dan pendeknya waktu operasi.Kata kunci: Free fatty acid, fatty acid steryl ester, modifikasi soxhlet ekstraksi, soybean oil deodorizer distillate, squalene, triacylglycerols","PeriodicalId":138501,"journal":{"name":"Jurnal Teknik Kimia Indonesia","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pemisahan squalene dan fatty acid sterol esters dari soybean oil deodorizer distillate\",\"authors\":\"Setiyo Gunawan, Yi Hsu Ju\",\"doi\":\"10.5614/jtki.2008.7.2.3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Depending on conditions in the refining process, soybean oil deodorizer distillate (SODD) contains 42-51% free fatty acids (FFA) and 16-25% triacylglycerol (TAG). Bioactive compounds such as tocopherols, free phytosterols, fatty acid steryl esters (FASE) and squalene also make up a significant portion of SODD. The efficient removal of FFA and TAG is a crucial step in the isolation and purification of bioactive compounds from SODD. In this work, a modified soxhlet extraction technique was developed and its optimal operation conditions were determined for the efficient removal of FFA and TAG from SODD. Starting with SODD that contains 3.53% FASE and 1.99% squalene, it was possible to obtain a fraction enriched with FASE (22.0%, recovery 91.3%) and squalene (8.63%, recovery 100%) by this modified soxhlet extraction under the following operation conditions: SODD/(silica gel) = 1/3 (w/w), extraction temperature = -6°C, number of extraction/h = 8.7. FFA remained in this FASE and squalene enriched fraction is 38.7% and there was no detectable TAG. The percentage removal of FFA and TAG for this FASE and squalene enriched fraction are 84.29% and 100%, respectively. The advantages of modified soxhlet extraction over open-column chromatography are that less solvent usage, larger sample size per batch and shorter operation time.Keywords: Fatty acid steryl ester; free fatty acid; modified soxhlet extraction; soybean oil deodorizer distillate; squalene; triacylglycerol AbstrakDalam proses pemurnian minyak kedelai, produk samping dari proses penghilangan bau (soybean oil deodorizer distillate, SODD) mengandung 42-52% free fatty acids (FFA) dan 18-28% triacylglycerols (TAG). Komponen bioaktif seperti tocopherols, free phytosterols, fatty acid steryl esters (FASE) dan squalene juga mempunyai kontribusi yang besar dalam komposisi SODD. Efisiensi penghilangan FFA dan TAG adalah langkah yang sangat penting dalam pemisahan dan pemurnian komponen bioaktif dari SODD. Dalam penelitian ini, modifikasi soxhlet ekstraksi telah ditemukan dan optimisasi kondisi operasi ditentukan berdasarkan efisiensi penghilangan FFA dan TAG dari SODD. Fraksi lemak non polar (nonpolar lipid fraction, NPLF) dengan kandungan FASE (22.0%, recovery 91.3%) dan squalene (8.63%, recovery 100%) telah diperoleh dengan menggunakan modifikasi soxhlet ektraksi dengan kondisi operasi: SODD/(silica gel) = 1/3 (w/w), dan suhu ekstraksi = -6°C. FFA dan TAG yang tersisa di NPLF secara berurutan adalah 38.7% dan 0%. Persentase penghilangan FFA dan TAG di NPLF secara berurutan adalah 84.29% and 100%. Keuntungan menggunakan modifikasi soxhlet ekstraksi dibandingkan dengan silica gel column chromatography adalah sedikitnya pelarut yang digunakan, besarnya sampel per unit batch yang digunakan, dan pendeknya waktu operasi.Kata kunci: Free fatty acid, fatty acid steryl ester, modifikasi soxhlet ekstraksi, soybean oil deodorizer distillate, squalene, triacylglycerols\",\"PeriodicalId\":138501,\"journal\":{\"name\":\"Jurnal Teknik Kimia Indonesia\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Jurnal Teknik Kimia Indonesia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5614/jtki.2008.7.2.3\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jurnal Teknik Kimia Indonesia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5614/jtki.2008.7.2.3","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
根据精制工艺条件的不同，大豆油脱臭馏出物(SODD)含有42-51%的游离脂肪酸(FFA)和16-25%的三酰甘油(TAG)。生物活性化合物，如生育酚、游离植物甾醇、脂肪酸甾酯(FASE)和角鲨烯也构成了SODD的重要部分。高效去除游离脂肪酸和TAG是从SODD中分离纯化生物活性化合物的关键步骤。本文研究了改进的索氏提取工艺，并确定了其最优操作条件，可有效去除SODD中的FFA和TAG。从含有3.53% FASE和1.99%角鲨烯的SODD开始，在SODD/(硅胶)= 1/3 (w/w)，提取温度= -6℃，提取次数/h = 8.7的操作条件下，改良索氏提取法可获得FASE(22.0%，回收率91.3%)和角鲨烯(8.63%，回收率100%)富集的馏分。FASE中残留FFA，角鲨烯富集部分为38.7%，TAG未检出。该FASE和角鲨烯富集部位对FFA和TAG的去除率分别为84.29%和100%。与开柱色谱法相比，改良索氏萃取法的优点是溶剂用量少，每批样品量大，操作时间短。关键词:脂肪酸甾酯;游离脂肪酸;改良索氏萃取法;大豆油脱臭馏出物;角鲨烯;【摘要】dalam加工pemurnian minyak kedelai，产品采样dari加工penghilangan bau(豆油脱臭馏出物，SODD)，原料为42-52%游离脂肪酸(FFA)和18-28%三酰甘油(TAG)。海参生物活性成分:生育酚、游离植物甾醇、脂肪酸甾酯(FASE)和角鲨烯。[footnoter.com] [footnoter.com] [footnoter.com] [footnoter.com]Dalam penelitian ini, modifikasi soxhlet ekstraksi telah ditemukan和optimisisi kondisi operasi ditentukan和berdasarkan efisiansan和tagdari SODD。非极性(非极性脂质分数，NPLF)登干坎顿干FASE(22.0%，回收率91.3%)丹角鲨烯(8.63%，回收率100%)丹干坎顿干修饰剂:SODD/(硅胶)= 1/3 (w/w)，丹苏胡ekstraksi = -6°C。FFA、TAG、yang、NPLF、secara和adalah分别为38.7%和0%。彭hilangan FFA, TAG, NPLF, secara berurutan adalah分别为84.29%和100%。keuntunan menggunakan modifikasi soxlet ekstraksi dibandingkan dengan硅胶柱色谱法adalah sedikitnya pelarut yang digunakan, bessanya样品每单位批次yang digunakan, dan pendeknya waktu operasi。Kata kunci:游离脂肪酸，脂肪酸steryl酯，改性索氏菌，大豆油脱臭馏出物，角鲨烯，三酰基甘油
Pemisahan squalene dan fatty acid sterol esters dari soybean oil deodorizer distillate
Depending on conditions in the refining process, soybean oil deodorizer distillate (SODD) contains 42-51% free fatty acids (FFA) and 16-25% triacylglycerol (TAG). Bioactive compounds such as tocopherols, free phytosterols, fatty acid steryl esters (FASE) and squalene also make up a significant portion of SODD. The efficient removal of FFA and TAG is a crucial step in the isolation and purification of bioactive compounds from SODD. In this work, a modified soxhlet extraction technique was developed and its optimal operation conditions were determined for the efficient removal of FFA and TAG from SODD. Starting with SODD that contains 3.53% FASE and 1.99% squalene, it was possible to obtain a fraction enriched with FASE (22.0%, recovery 91.3%) and squalene (8.63%, recovery 100%) by this modified soxhlet extraction under the following operation conditions: SODD/(silica gel) = 1/3 (w/w), extraction temperature = -6°C, number of extraction/h = 8.7. FFA remained in this FASE and squalene enriched fraction is 38.7% and there was no detectable TAG. The percentage removal of FFA and TAG for this FASE and squalene enriched fraction are 84.29% and 100%, respectively. The advantages of modified soxhlet extraction over open-column chromatography are that less solvent usage, larger sample size per batch and shorter operation time.Keywords: Fatty acid steryl ester; free fatty acid; modified soxhlet extraction; soybean oil deodorizer distillate; squalene; triacylglycerol AbstrakDalam proses pemurnian minyak kedelai, produk samping dari proses penghilangan bau (soybean oil deodorizer distillate, SODD) mengandung 42-52% free fatty acids (FFA) dan 18-28% triacylglycerols (TAG). Komponen bioaktif seperti tocopherols, free phytosterols, fatty acid steryl esters (FASE) dan squalene juga mempunyai kontribusi yang besar dalam komposisi SODD. Efisiensi penghilangan FFA dan TAG adalah langkah yang sangat penting dalam pemisahan dan pemurnian komponen bioaktif dari SODD. Dalam penelitian ini, modifikasi soxhlet ekstraksi telah ditemukan dan optimisasi kondisi operasi ditentukan berdasarkan efisiensi penghilangan FFA dan TAG dari SODD. Fraksi lemak non polar (nonpolar lipid fraction, NPLF) dengan kandungan FASE (22.0%, recovery 91.3%) dan squalene (8.63%, recovery 100%) telah diperoleh dengan menggunakan modifikasi soxhlet ektraksi dengan kondisi operasi: SODD/(silica gel) = 1/3 (w/w), dan suhu ekstraksi = -6°C. FFA dan TAG yang tersisa di NPLF secara berurutan adalah 38.7% dan 0%. Persentase penghilangan FFA dan TAG di NPLF secara berurutan adalah 84.29% and 100%. Keuntungan menggunakan modifikasi soxhlet ekstraksi dibandingkan dengan silica gel column chromatography adalah sedikitnya pelarut yang digunakan, besarnya sampel per unit batch yang digunakan, dan pendeknya waktu operasi.Kata kunci: Free fatty acid, fatty acid steryl ester, modifikasi soxhlet ekstraksi, soybean oil deodorizer distillate, squalene, triacylglycerols
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