{"title":"体外维持中枢组织以进行后续药理学评估:一种简单而廉价的灌注室","authors":"Erick W. Black, Joan M. Lakoski","doi":"10.1016/0160-5402(91)90028-4","DOIUrl":null,"url":null,"abstract":"<div><p>A new simple, inexpensive holding chamber is described for maintaining brain slices in a viable condition for long periods of time. The advantages of its superfusion-type operation and application of this chamber to in vitro electrophysiologic studies of the pharmacologie action of serotonin in the midbrain dorsal raphe nucleus are discussed.</p></div>","PeriodicalId":16819,"journal":{"name":"Journal of pharmacological methods","volume":"25 4","pages":"Pages 285-289"},"PeriodicalIF":0.0000,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0160-5402(91)90028-4","citationCount":"3","resultStr":"{\"title\":\"Maintenance of cns tissues in vitro for subsequent pharmacologie evaluation: A simple and inexpensive superfusion chamber\",\"authors\":\"Erick W. Black, Joan M. Lakoski\",\"doi\":\"10.1016/0160-5402(91)90028-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A new simple, inexpensive holding chamber is described for maintaining brain slices in a viable condition for long periods of time. The advantages of its superfusion-type operation and application of this chamber to in vitro electrophysiologic studies of the pharmacologie action of serotonin in the midbrain dorsal raphe nucleus are discussed.</p></div>\",\"PeriodicalId\":16819,\"journal\":{\"name\":\"Journal of pharmacological methods\",\"volume\":\"25 4\",\"pages\":\"Pages 285-289\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0160-5402(91)90028-4\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of pharmacological methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0160540291900284\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of pharmacological methods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0160540291900284","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Maintenance of cns tissues in vitro for subsequent pharmacologie evaluation: A simple and inexpensive superfusion chamber
A new simple, inexpensive holding chamber is described for maintaining brain slices in a viable condition for long periods of time. The advantages of its superfusion-type operation and application of this chamber to in vitro electrophysiologic studies of the pharmacologie action of serotonin in the midbrain dorsal raphe nucleus are discussed.
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