T. López-Gutiérrez, B. Sarabia-Alcocer, E. Gutiérrez-Alcántara, B. Aké-Canché
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引用次数: 0
摘要
研究了从海洋细菌mojavensis Bacillus (MC3B-22)培养物中分离纯化的脂肽对炭疽病菌(Colletotrichum gloeosporioides Penz & Sacc vars minor Simmonds)的抑菌活性。结果表明,与酸沉法(0.3173 g/L)相比,硫酸铵提取法提取生物表面活性剂的量最大,为3.1243 g/L。103个分生孢子/mL的粗提物对炭黑病菌无抑制作用,硫酸铵半纯化提物对炭黑病菌的最低抑制浓度为12.5µg/mL。综上所述,生物表面活性剂的最佳提取工艺为40%硫酸铵,以甲醇为半纯化溶剂,对gloeosporioides的最小抑制浓度为25µg/mL。
Lipopeptide produced by the bacteria Bacillus mojavensis with activity against the phytopathogenic fungus Colletotrichum gloeosporioides Penz & Sacc var. Minor Simmonds
The objective of this work was to determine the antifungal activity against Colletotrichum gloeosporioides Penz & Sacc var. minor Simmonds of the lipopeptide(s) isolated and purified from the culture of the marine bacterium Bacillus mojavensis (MC3B-22). The results will show that the extraction method with ammonium sulfate was the one that extracted the largest amount of biosurfactants with a yield of 3.1243 g/L compared to the acid precipitation method (0.3173 g/L). The crude extract in the presence of 103 conidia/mL of the fungus Colletotrichum gloeosporioides did not present inhibition, but the semi-purified extract obtained with ammonium sulfate reached a minimum inhibitory concentration of 12.5 µg/mL. In conclusion, the optimal method for extracting the biosurfactant was 40% ammonium sulfate, with methanol being a suitable solvent to semi-purify and obtain a minimum inhibitory concentration of 25 µg/mL against C. gloeosporioides.