Detection of total flavonoid, antioxidant activity and HPLC analysis of methanolic extract of Silybum marianum seeds

Silybum marianum considered as an ancient herb promising plants. In this work, fhese study was design to assess total flavonoids, anti-oxidant and HPLC analysis of Silybum marianum plant extract by methanol in vitro. Total flavonoids had been measured in the extract that about 201.6667±1.52753 mg/ml. anti-oxidant activity of methanolic plant extract Silybum marianum in vitro was estimated by assesment of reductive ability, in concentrations that be tested (0.02, 0.04, 0.08, 0.16, 0.32 and 0.64 mg/ml) , the absorbance had been increased significantly , results of HPLC analysis of plant contain (Ascorbic acid ,Gallic acid Catechin, Rutin, Quercetin, Apigeninand Kaemp ferol) in concentration (0.839, 0.0918, 0.628, 2.989, 0.010, 0.0680, 0.228) ppm respectivily.