Evaluation of the effect of obesity on oxidative stress with thiol/disulphide balance

Aims: As the body mass index (BMI) increases, the percentage of body fat increases and oxidative stress increases accordingly. This change can be determined by looking at the thiol/disulphide balance which is a biochemical test. We aim to emphasize the importance of oxidative stress due to obesity. Thus, by protecting obese individuals from oxidative stress (with antioxidant support treatments, etc.), studies to minimize the impact of obesity can be opened the front. Methods: The study group of our study was selected from 18-55-year-old female obese or overweight female patients who applied to Kırıkkale University Medical Faculty Internal Medicine and Endocrinology (Obesity) Polyclinics for slimming. Acontrol group consisting of healthy and normal weighted women who were in the same age range and applied to our hospital for general control were included in the study. According to BMI; groups consisting of 45 women, normal weight (18,5-24,9 kg/m²), overweight (25-29,9 kg/m²), stage 1 (mild obese; 30-34,9 kg/m²), stage 2 (moderately obese; 35-39,9 kg/m²) and stage 3 (morbid obese; 40-49,9 kg/m²), were formed. Participants were premenopausal women with no additional disease. Demographic data and routine investigations were obtained from the hospital system. Blood samples were studied with thiol/disulphide balance measurement tests. Results: We analyzed the five groups that were graded as normal weight, overweight, obese (mild, moderate, morbid) and the positive correlation between BMI and BC and BFP was significant in all groups (r =0.936, p<0.001; r =0.857, p<0.001, respectively). In all groups, the levels of native thiol and total thiol were decreased as BMI increased. There is a relatively lower difference between the normal thiol and total thiol levels of the normal weight and overweight group; there was a significant decrease in these values when passing from overweight to any stage of obesity. Negative correlation between all groups with native thiol (r =-0.473, p<0.001), total thiol (r =-0.472, p<0.001) and SH/total SH values (r =-0.296, p<0.001) were significant. The positive correlation between SS/SH (r = 0.296, p<0.001) and SS/total SH (r =0.296, p<0.001) was significant in all groups. The positive correlation between disulphide and all groups (r =0.103, p=0.25) was not significant. Conclusion: Increased fat tissue inflammation in obesity is associated with oxidative stress. Thiol-containing organic compounds are antioxidants for defense against oxidative stress. Measures should be taken in the early period to reduce oxidative stress in the management of obesity.