基于数字微流控生物芯片中多个液滴的单故障检测技术

M. Majumder, Uttam Dolai, Arindam Bhattacharya
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引用次数: 8

摘要

微流控生物芯片促进了生物医学操作或安全关键应用的革命性改进,如临床诊断,平行DNA测序,毒性监测,免疫测定,空气或水质监测,食品安全检测等。但由于电极的缺陷,它在流体操作中遇到了很大的挫折。在本文中,我们提出了一种新的有效的技术来检测生物芯片中的单个故障并识别故障位置。除此之外,它还可以计算生物芯片无故障时的遍历时间。微阵列的遍历是通过特殊类型的移动模式RDRD (right - down - right - down)和DRDR (Down-Right-Down-Right)进行左、右对角电极遍历的中间细胞和边缘扫描,通过移动测试液滴顺时针方向遍历边界细胞和边缘。如果检测到故障,那么所提出的技术也可以通过回溯液滴来定位故障。仿真结果表明,该方法是有效的,在故障检测和无故障生物芯片遍历时间计算方面比现有方法有了显著的改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An efficient novel single fault and its location detection technique using multiple droplets in a Digital Microfluidic Biochip
Microfluidic biochip has facilitated a revolutionary improvement in biomedical operation or safety critical application like clinical diagnosis, parallel DNA sequencing, toxicity monitoring, immunoassay, air or water quality monitoring, food safety testing etc. But it has faced a major setback from malfunction in fluidic operation due to the defect in the electrodes. In this paper, we are proposing a novel and efficient technique for detecting a single fault and identifying the fault location within the biochip. Along with that it can also calculate the traversal time if the biochip is fault free. The traversal of the microarray is carried out by scanning the intermediate cells and the edges by special types of movement pattern RDRD (Right-Down-Right-Down) and DRDR (Down-Right-Down-Right) for left and right diagonal electrode traversal respectively and the boundary cells and edges are traversed in clockwise direction by moving the test droplets. If a fault is detected then the proposed technique also locates it by backtracking the droplet. The simulated result suggests that the proposed technique is efficient and represents significant improvement in fault detection and calculating traversal time of a fault free biochip over existing methods.
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