用于水生环境中微生物ATP定量分析的集成原位分析仪的研制

2007 Symposium on Underwater Technology and Workshop on Scientific Use of Submarine Cables and Related Technologies Pub Date : 2007-04-17 DOI:10.1109/UT.2007.370802

T. Fukuba, N. Fukuzawa, L. S. Glutz, A. Miyaji, T. Fujii

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引用次数: 4

摘要

本研究研制了一种微型集成的微生物ATP原位定量分析装置。该装置可以在微流控芯片中进行细胞外ATP消除、细胞裂解和ATP定量。一种微通道的模式已被优化，以测量荧光素-荧光素酶反应引起的发光。通过对所研制的微流控装置的评价，成功地定量了10 nM ~ 100 muM的ATP。微流控装置还对环境样品和细菌培养基进行了分析。针对ATP定量分析装置的现场标定，制作了一种基于笼式ATP在线标定元件的样机，并对其进行了评价。

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Development of an Integrated In Situ Analyzer for Quantitative Analysis of Microbial ATP in Aquatic Environments

An integrated and miniaturized device for in situ quantitative analysis of microbial ATP was developed in this study. The device can perform extracellular ATP elimination, cell lysis and ATP quantification in a microfluidic chip. A pattern of a microchannel has been optimized to measure luminescence caused by luciferin-luciferase reaction. As a result of evaluation of the developed microfluidic device, 10 nM to 100 muM of ATP was successfully quantified. Environmental samples and bacterial culture media were also analyzed by the microfluidic device. Towards in situ calibration of the ATP quantitative analysis device, a prototype of caged ATP based online calibration component was also fabricated and evaluated.

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2007 Symposium on Underwater Technology and Workshop on Scientific Use of Submarine Cables and Related Technologies

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