测试黑醋酸抗真菌活性对菌根生长

Eva Agustina, Funsu Andiarna, Irul Hidayati, Vindi Fristy Kartika
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引用次数: 1

摘要

一种起抗真菌作用的植物是将大蒜注入黑或黑大蒜的产品中。这项研究的目的是确定黑莓菌提取物对堪培拉真菌生长的抗菌活性。这项研究的方法是加热大蒜35天在65°C的温度下才能得到黑大蒜。黑大蒜是用甲烷溶剂用人工合成方法提取的。根据区抑制、最低浓度抑制和最低杀伤浓度对抗菌活性进行测试。区域抑制试验与提取100%、90%和80%的浓度变化。具有良好消化区价值的浓度将继续进行KHM测试和KBM,减少到1到5。研究表明,100%浓度的黑蒜味萃取区有最好的消化区是3.15毫米。KHM最佳试验是在第5次稀释中获得的,OD分数最低0.460。在第5次稀释中对黑醋栗提取物的KBM测试表明,真菌C.阿尔比坎人的菌落还在生长,所以可以说,黑大蒜提取物只能抑制真菌的生长,但不能杀死真菌C.阿尔比坎人。关键词:抗真菌药;黑大蒜;用黑大蒜测试研究坎地达菌的生长。一种像抗铅酸盐一样的植物,其成分是无缝的黑色大蒜。这项研究的目的是确定黑黄瓜extract antifungal activity反对坎迪达阿尔比坎人。这个研究方法》是heated大蒜为35天在65°C的温度。黑大蒜是用甲烷溶剂提取的maxy method方法。抑制抑制试验,抑制抑制最小,杀死集中(MBC)。区域注射与变异extract concentrations 100%, 90%和80%。麦克风和MBC测试在1到5之外。结果显示,黑醋酸盐区的inhibition出口具有100%的集中,3.15毫米。最佳的麦克风测试是5 . 0.460分被拒绝的损失控制。在展区外第五区进行的MBC测试,阿尔比坎人的fungal殖民地仍在发展,所以可以肯定地说,黑黄瓜extract只对fungal growth感兴趣,但不能杀死C. albicans真菌。安装:antifungal;黑大蒜;maceration
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Uji aktivitas antijamur ekstrak black garlic terhadap pertumbuhan jamur Candida albicans
Salah satu tanaman yang berfungsi sebagai  antijamur  adalah bawang  putih yang diinovasikan menjadi produk bawang yang berwarna hitam atau black garlic. Penelitian ini bertujuan untuk mengetahui aktivitas antijamur ekstrak black barlic terhadap pertumbuhan jamur Candida albicans. Metoda dalam penelitian ini adalah bawang putih dipanaskan selama 35 hari pada suhu 65°C untuk mendapatkan black garlic. Black garlic diekstraksi dengan metode maserasi menggunakan pelarut methanol. Uji aktivitas antibakteri berdasarkan uji zona hambat, konsentrasi hambat minimal (KHM) dan konsentrasi bunuh minimal (KBM). Uji zona hambat dengan variasi konsentrasi ekstrak 100%, 90% dan 80%. Konsentrasi dengan nilai zona hambat yang baik akan dilanjutkan ke uji KHM dan KBM dengan pengenceran ke 1 sampai 5. Hasil penelitian menunjukkan bahwa zona hambat ekstrak Black Garlic dengan konsentrasi 100% memiliki zona hambat paling bagus yaitu 3,15 mm. Uji KHM optimum diperoleh pada pengenceran ke-5 dengan nilai OD terendah 0,460. Uji KBM dari ekstrak black garlic pada pengenceran ke-5 menunjukkan koloni jamur C. albicans masih tumbuh, sehingga dapat dikatakan bahwa ekstrak black garlic hanya mampu menghambat pertumbuhan jamur namun tidak bisa membunuh jamur C. albicans. Kata kunci: antijamur; black garlic; maserasi  ABSTRACTActivity test of black garlic extract against the growth of the fungi Candida albicans. One plant that functions as an antifungal is garlic which is innovated into black garlic. The aim of this study is to indentify black garlic extract antifungal activity against of the Candida albicans. The method in this research was heated garlic for 35 days at temperature of 65°C. Black garlic was extracted by maceration method using methanol solvent. Antibacterial activity test based on inhibition zone test, minimal inhibitory concentration (MIC) and minimal kill concentration (MBC). Inhibition zone test with various extract concentrations of 100%, 90% and 80%. Concentrations with good inhibition zone values will be continued to the MIC and MBC tests with dilutions 1 to 5. The results showed that the inhibition zone of black garlic extract with a concentration of 100%, 3.15 mm. The optimum MIC test was obtained at the 5 dilution with the lowest OD value of absorbance 0.460. The MBC test of the black garlic extract at the 5 dilution showed C. albicans fungal colonies were still growing, so it can be said that the black garlic extract was only able to inhibit fungal growth but could not kill C. albicans fungi. Keywords: antifungal; black garlic; maceration
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