几种杆菌兼容性作为一种生物肥料

Yun Sondang, Muflihayati Muflihayati, K. Anty, R. Siregar
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摘要

细菌是生物有机肥生产过程中潜在的助剂。生物有机肥过程中有机物的分解速率取决于功能菌的作用、有机物的种类和微环境。接种到生物有机肥物质中的细菌之间必须协同作用,使其作为生物活化剂的作用更有效。为了获得一种高效的生物有机肥,必须对用作生物活化剂的分离菌进行相容性实验。研究的目的是获得有关细菌分离株相容性的信息。该研究于2022年7月至8月在西苏门答腊岛Payakumbuh州立农业理工学院的植物保护实验室进行。研究方法首先在NA培养基上培养蜡样芽孢杆菌、枯草芽孢杆菌、苏云金芽孢杆菌的复青菌分离株,然后在NA和King’s B培养基上采用双重培养法进行相容性实验,共12个处理(单处理和联合处理),3个重复。3种芽孢杆菌在NA培养基上的相容性指数(IK)为0.67-1,在King’s B培养基上的相容性指数为0.90-1.00,亲和性实验结果表明,3种芽孢杆菌之间的生长不相互抑制,可以同时作为生物有机肥过程中的生物激活剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
KOMPATIBILITAS BEBERAPA SPESIES Bacillus SEBAGAI BIOAKTIVATOR PUPUK ORGANIK HAYATI
Bacteria is a potential agent in biological organic fertilizer”s process. The rate of decomposition of organic matter in biological organic fertilizers process depends on the role of funtional bacteria, the type of organic matter and the microenvironment.  Bacteria that are inoculated into biological organic fertilizer’s matter must be synergistic among them, so that their role as as bioactivators more efficient.  A compatibility experiment among the bacteria isolates used as bioactivators have to be done in order to  obtain an effective and efficient biological organic fertilizer. The objevctive of the research is to obtain information on the compatible nature of the bacterial isolates. The research was conducted at the Plant Protection Laboratory of the Payakumbuh State Agricultural Polytechnic, West Sumatra from July to August 2022. The research method began with the rejuvenation bacterial isolates of Bacillus cereus, Bacillus subtilis, Bacillus thuringiensis on NA media, then continued with a compatibility experiment  using the dual culture method on NA and King’s B medium, so there were a total of 12 treatments (single and combination) with three replications. The results of the compatibility experiment on the three Bacillus bacteria did not form a halo inhibition zone and were compatible with the compatibility index value (IK) 0.67-1 on NA medium and 0.90-1.00 on King’s B medium, meaning that the growth between the three Bacillus bacteria did not inhibit each other, so that all Bacillus bacteria could be used as a bioactivator simultaneously in biological organic fertilizer’s process.
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