利用简单序列重复标记分析茄子群体遗传多样性

M. Faizan, B. N. Harish Babu, B. Fakrudin, D. Lakshmana, M. Rakshith
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引用次数: 0

摘要

茄子(Solanum melongena L.)是世界上第三重要的茄类蔬菜,也是分布在世界地理区域内物种最多样化的蔬菜。利用23个SSR标记对54个茄子基因型(5个亚群体)的遗传多样性进行了分析。茄子5个亚群体的分子变异分析表明,群体内变异率为90.67%,群体间变异率为9.34%。SSR标记分析结果显示:平均观察杂合度(0.216)、平均期望杂合度(0.496)、香农信息指数(0.879)、平均不同等位基因数(3.209)、平均有效等位基因数(2.535)、固定指数(0.649);此外,系统发育分析明确了遗传差异个体的分类,其中最多样化的集群是集群1 (C1),特别是野生品种被归为集群5 (C5)。研究结果可为茄子育种和种质资源保护提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dissection of genetic diversity present in eggplant populations using simple sequence repeat markers
Eggplant (Solanum melongena L.) is the third most important solanaceous vegetable and most diversified within species spread across the world-geographical area. A study was conducted to assess the genetic diversity among the selected fifty-four eggplant genotypes (sub-categorized into five sub-population) using twenty-three SSR markers. The Analysis of Molecular Variance among the five sub-population of eggplant revealed the existence of 90.67% variation within populations and 9.34% variation among populations. The SSR markers analysis revealed important locus-wise information like mean Observed-Heterozygosity (0.216), mean Expected-Heterozygosity (0.496), Shannon’s Information Index (0.879), mean number of different alleles (3.209), mean number of effective alleles (2.535), Fixation-Index (0.649). Further, Phylogenetic-analysis clearly categorize genetically distinct individuals in which the most diversified clusters was cluster-1 (C1) out of total of five clusters and especially, wild cultivars were grouped into cluster-5 (C5). The obtained results can be used in eggplant breeding and germplasm conservation in a resourceful manner.
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