{"title":"金盏花和金盏花水提物的植物化学筛选、金属离子含量及其对动物实验模型抗银屑病活性的影响","authors":"Kuntal Das, S. Deb, Tejaswini Karanth","doi":"10.4274/tjps.44265","DOIUrl":null,"url":null,"abstract":"Objective The aim of this study is to evaluate the influence of metal ions present in soil as well as in the leaves samples of Calendula officinalis and Phlebodium decumanum for the treatment of psoriasis. Methods Looking at the objective, soils and leaves samples were estimated for metal ions by atomic absorption spectrophotometer to determine the influence in anti-psoriatic activity. Thereafter Imiquimod-induced dermatitis lesions were created in grouped mouse. Two plant extracts (aqueous) separately as well as in combinations and standard Retino-A (0.05%) were used. Psoriasis severity index (PSI) was evaluated by phenotypic (redness, erythema, and scales) and histological features (epidermal thickness). Further content of phytochemicals in terms of extract was correlated with the effect of psoriasis activity. Results: We observed redness, erythema and scales and the histological features and found a progressive reduction (P < 0.05) in the severity of psoriatic lesions (redness, erythema, and scales) from day 7 to 21st day and decreased epidermal thickness in animals treated with combined extracts at the dose of 200 mg/kg b.w. Furthermore plant samples procured from Nandi Hill’s, Bangalore, showed better uptake of metals with respect to Fe (2.05 mg/kg), Cu (0.78 mg/kg) and Zn (1.12 mg/kg) which showed positive impact on procurement of maximum amount of extracts that further correlated with the activity, indicated significant reduction of psoriatic lesions. un co rr ct d p roo f Conclusion Results revealed that significant dose dependent antipsoriasis activity of combined aqueous extracts of Calendula officinalis and Phlebodium decumanum as well as metal ions are also had impact on the procurement of extracts and said activity.","PeriodicalId":193717,"journal":{"name":"The Turkish Journal of Pharmaceutical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phytochemical screening, metallic ion content and from its impact on Anti psoriasis activity of Calendula officinalis and Phlebodium decumanum aqueous leaf extracts in animal experiment model\",\"authors\":\"Kuntal Das, S. Deb, Tejaswini Karanth\",\"doi\":\"10.4274/tjps.44265\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective The aim of this study is to evaluate the influence of metal ions present in soil as well as in the leaves samples of Calendula officinalis and Phlebodium decumanum for the treatment of psoriasis. Methods Looking at the objective, soils and leaves samples were estimated for metal ions by atomic absorption spectrophotometer to determine the influence in anti-psoriatic activity. Thereafter Imiquimod-induced dermatitis lesions were created in grouped mouse. Two plant extracts (aqueous) separately as well as in combinations and standard Retino-A (0.05%) were used. Psoriasis severity index (PSI) was evaluated by phenotypic (redness, erythema, and scales) and histological features (epidermal thickness). Further content of phytochemicals in terms of extract was correlated with the effect of psoriasis activity. Results: We observed redness, erythema and scales and the histological features and found a progressive reduction (P < 0.05) in the severity of psoriatic lesions (redness, erythema, and scales) from day 7 to 21st day and decreased epidermal thickness in animals treated with combined extracts at the dose of 200 mg/kg b.w. Furthermore plant samples procured from Nandi Hill’s, Bangalore, showed better uptake of metals with respect to Fe (2.05 mg/kg), Cu (0.78 mg/kg) and Zn (1.12 mg/kg) which showed positive impact on procurement of maximum amount of extracts that further correlated with the activity, indicated significant reduction of psoriatic lesions. un co rr ct d p roo f Conclusion Results revealed that significant dose dependent antipsoriasis activity of combined aqueous extracts of Calendula officinalis and Phlebodium decumanum as well as metal ions are also had impact on the procurement of extracts and said activity.\",\"PeriodicalId\":193717,\"journal\":{\"name\":\"The Turkish Journal of Pharmaceutical Sciences\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2018-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Turkish Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4274/tjps.44265\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Turkish Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4274/tjps.44265","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的探讨金盏菊、金盏金盏花叶片和土壤中金属离子对银屑病的治疗作用。方法采用原子吸收分光光度法测定土壤和叶片样品中金属离子含量,测定其抗银屑病活性的影响。随后,建立了咪喹莫德致小鼠皮炎病变。分别使用两种植物提取物(水)、联合使用和标准维甲酸a(0.05%)。银屑病严重指数(PSI)通过表型(发红、红斑和鳞片)和组织学特征(表皮厚度)来评估。提取物中植物化学物质的进一步含量与银屑病活性的影响相关。结果:我们观察了红肿、红斑、鳞片和组织学特征,并发现从第7天到第21天,银屑病病变(红肿、红斑和鳞片)的严重程度逐渐降低(P < 0.05),表皮厚度减少,剂量为200 mg/kg b.w的联合提取物治疗的动物。此外,从班加罗尔的Nandi Hill 's获得的植物样品显示出更好的金属吸收,其中铁(2.05 mg/kg)。铜(0.78 mg/kg)和锌(1.12 mg/kg)对最大提取量的采购有积极影响,与活性进一步相关,表明银屑病病变显著减少。结论金盏菊、金盏黄联合水提物的抗银屑病活性及金属离子对提取物的获取及抗银屑病活性均有显著的剂量依赖性。
Phytochemical screening, metallic ion content and from its impact on Anti psoriasis activity of Calendula officinalis and Phlebodium decumanum aqueous leaf extracts in animal experiment model
Objective The aim of this study is to evaluate the influence of metal ions present in soil as well as in the leaves samples of Calendula officinalis and Phlebodium decumanum for the treatment of psoriasis. Methods Looking at the objective, soils and leaves samples were estimated for metal ions by atomic absorption spectrophotometer to determine the influence in anti-psoriatic activity. Thereafter Imiquimod-induced dermatitis lesions were created in grouped mouse. Two plant extracts (aqueous) separately as well as in combinations and standard Retino-A (0.05%) were used. Psoriasis severity index (PSI) was evaluated by phenotypic (redness, erythema, and scales) and histological features (epidermal thickness). Further content of phytochemicals in terms of extract was correlated with the effect of psoriasis activity. Results: We observed redness, erythema and scales and the histological features and found a progressive reduction (P < 0.05) in the severity of psoriatic lesions (redness, erythema, and scales) from day 7 to 21st day and decreased epidermal thickness in animals treated with combined extracts at the dose of 200 mg/kg b.w. Furthermore plant samples procured from Nandi Hill’s, Bangalore, showed better uptake of metals with respect to Fe (2.05 mg/kg), Cu (0.78 mg/kg) and Zn (1.12 mg/kg) which showed positive impact on procurement of maximum amount of extracts that further correlated with the activity, indicated significant reduction of psoriatic lesions. un co rr ct d p roo f Conclusion Results revealed that significant dose dependent antipsoriasis activity of combined aqueous extracts of Calendula officinalis and Phlebodium decumanum as well as metal ions are also had impact on the procurement of extracts and said activity.