{"title":"~(60)Co γ辐照可增强大鼠脑组织中GAP-43 mRNA的表达","authors":"B. Su, W. Cai, Cheng-gang Zhang, B. Perbal","doi":"10.3760/CMA.J.ISSN.0254-5098.2001.02.003","DOIUrl":null,"url":null,"abstract":"Objective\\ To study the relationship between the expression of GAP\\|43 mRNA and nerve regeneration in rat brain after 60 Co γ\\|irradiation. Methods\\ Wistar rats were subjected to whole\\|body irradiation with 8 Gy 60 Co γ\\|rays.The expression of GAP\\|43 was detected by in situ hybridization histochemistry using Dig\\|cRNA probe. Results\\ It was found that the expression of GAP\\|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ\\|irradiation.The peak of GAP\\|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion\\ Enhanced expression of GAP\\|43 mRNA after 60 Co γ\\|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.","PeriodicalId":374575,"journal":{"name":"Chinese Journal of Radiation Mediation and Protection","volume":"21 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"~(60)Co gamma-irradiation enhances expression of GAP-43 mRNA in rat brain\",\"authors\":\"B. Su, W. Cai, Cheng-gang Zhang, B. Perbal\",\"doi\":\"10.3760/CMA.J.ISSN.0254-5098.2001.02.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective\\\\ To study the relationship between the expression of GAP\\\\|43 mRNA and nerve regeneration in rat brain after 60 Co γ\\\\|irradiation. Methods\\\\ Wistar rats were subjected to whole\\\\|body irradiation with 8 Gy 60 Co γ\\\\|rays.The expression of GAP\\\\|43 was detected by in situ hybridization histochemistry using Dig\\\\|cRNA probe. Results\\\\ It was found that the expression of GAP\\\\|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ\\\\|irradiation.The peak of GAP\\\\|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion\\\\ Enhanced expression of GAP\\\\|43 mRNA after 60 Co γ\\\\|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.\",\"PeriodicalId\":374575,\"journal\":{\"name\":\"Chinese Journal of Radiation Mediation and Protection\",\"volume\":\"21 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2001-03-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chinese Journal of Radiation Mediation and Protection\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2001.02.003\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chinese Journal of Radiation Mediation and Protection","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3760/CMA.J.ISSN.0254-5098.2001.02.003","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
目的研究60 Co γ辐照后大鼠脑组织GAP 43 mRNA表达与神经再生的关系。方法采用8 Gy 60 Co γ射线对Wistar大鼠进行全身照射。采用Dig\ \ cRNA探针原位杂交组织化学方法检测GAP\ \ 43的表达。结果8 Gy 60 Co γ辐照1周后，大鼠大脑皮层、尾状核、壳核、苍白球、丘脑和下丘脑的GAP 43 mRNA表达增加。GAP\|43 mRNA表达在第四周达到峰值，随后开始下降，但仍保持在高于正常水平。7周后降至较低水平。结论60 Co γ辐照后大鼠脑组织GAP 43 mRNA表达增强与神经再生和突触重建有关。
~(60)Co gamma-irradiation enhances expression of GAP-43 mRNA in rat brain
Objective\ To study the relationship between the expression of GAP\|43 mRNA and nerve regeneration in rat brain after 60 Co γ\|irradiation. Methods\ Wistar rats were subjected to whole\|body irradiation with 8 Gy 60 Co γ\|rays.The expression of GAP\|43 was detected by in situ hybridization histochemistry using Dig\|cRNA probe. Results\ It was found that the expression of GAP\|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ\|irradiation.The peak of GAP\|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion\ Enhanced expression of GAP\|43 mRNA after 60 Co γ\|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
