用高粱(高粱双色)培养基制备木质素降解真菌接种菌及其对竹叶堆肥质量的影响

Nadya Febri Harlifia, Bambang Irawan, S. Farisi, Suratman
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引用次数: 1

摘要

竹子是一种生长在印度尼西亚的植物。到目前为止,人们只使用茎,把叶子扔掉。结果,这些丰富的竹叶变成了废物。竹叶废弃物木质素含量为19.8 ~ 26.6%,长期可转化为堆肥。竹叶凋落物的堆肥过程可以使用激活剂加速,例如接种真菌Geotrichum sp.,它具有木质素分解作用,可以将竹叶废弃物中含有的木质素复合化合物分解成更简单的化合物。高粱含有约6.5-7.9%的纤维。高粱种子纤维含量高,可作为真菌接种的生长培养基。本试验的目的是确定高粱作为土曲菌的接种培养基的效果以及接种对堆肥质量的影响。该研究于2019年8月至12月在尤尼拉大学数学与自然科学学院生物系微生物实验室进行。本研究采用完全随机设计(CRD)方法,设对照(竹叶凋落物)、P1(竹叶凋落物+ 1%接种量)、P2(竹叶凋落物+ 1.5%接种量)和P3(竹叶凋落物)4个处理。+ 2%接种量),每次处理重复3次。采用菌落形成单位(Colony Forming Unit, CFU)计算接种量和堆肥量,观察孢子数和孢子活力。通过分析C、N、P含量和C/N比来确定堆肥的质量。研究结果表明,以高粱为培养基接种木质素降解真菌Geotrichum sp.,孢子活力为74.18%,添加1.5%接种量(P2)的竹叶堆肥质量最佳。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.)
Bamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.)
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