乳酸乳球菌的呼吸作用是一种避免氧化应激的策略,它受血红素状态的调节

B. Cesselin, Aurélie Derré-Bobillot, Annabelle Fernandez, G. Lamberet, D. Lechardeur, Yuji Yamamoto, M. Pedersen, C. Garrigues, P. Gaudu
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引用次数: 8

摘要

乳酸菌(LAB),如乳酸菌,传统上被认为是专性发酵菌,因为即使在好氧作用中,它们也利用糖降解进行底物水平的磷酸化,即ATP的产生。然而,最近的研究表明,这种细菌和其他一些LAB能够激活血红素依赖性细胞色素氧化酶(CydAB),从而进行呼吸代谢。然而,呼吸链激活只有在细胞能够接触到环境中的血红素(对某些LAB来说,还包括甲基萘醌)时才被允许,因为它们不能合成这些化合物。呼吸作用增加了生物量产量,延长了储存细胞的长期存活期。呼吸的这些好处有不同的解释:i)呼吸链活动消耗氧气,限制有毒活性氧的形成。ii)呼吸链产生pH梯度,这可能通过H+-ATP酶活性增加ATP的产生。iii)呼吸代谢减少乳酸的产生,限制酸应激。然而,LAB必须应对血红素毒性。虽然血红素具有明显的代谢益处,但必须严格控制细胞内的游离血红素池,以防止对DNA等大分子的损害。在乳酸菌中,一个由atp酶(YgfA)和渗透酶(YgfB)组成的潜在外排泵系统对外源血红素的反应是高度诱导的。有趣的是,ygfA和ygfB基因与TetR家族的潜在调节因子ygfC位于一个操纵子中。我们的研究表明,ygfCBA操纵子参与调节游离血红素水平,并受YgfC调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Respiration, a strategy to avoid oxidative stress in Lactococcus lactis, is regulated by the heme status
Lactic acid bacteria (LAB) species like L. lactis are traditionally considered as obligate fermentative bacteria because even in aerobiosis they use sugar degradation for substrate-level phosphorylation, i.e. ATP production. However, recent studies revealed that this bacterium and some other LAB are capable of activating a heme-dependent cytochrome oxidase (CydAB) and thus undergo a respiration metabolism. Nevertheless, respiratory chain activation is allowed only when cells have access to heme (and additionally menaquinone for some LAB) in the environment because they cannot synthesize these compounds. Respiration increases the biomass yield and extends the long term survival of stored cells. These benefits of respiration are explained in different ways: i) Respiratory chain activity consumes oxygen, limiting the formation of toxic reactive oxygen species. ii) Respiratory chain generates a pH gradient, which potentially increases ATP production via H+-ATPase activity. iii) Respiration metabolism decreases lactic acid production, limiting acid stress. However, LAB have to cope with heme toxicity. Although heme has clear metabolic benefits the intracellular pool of free heme must be stringently controlled to prevent damage to macromolecules like DNA. In L. lactis, a potential efflux pump system, consisting of an ATPase (YgfA) and a permease (YgfB), is specifically highly induced in response to exogenous heme. Interestingly, the ygfA and ygfB genes are in an operon with ygfC, a potential regulator of the TetR family. Our studies implicate the ygfCBA operon is involved in modulating the free heme level and is regulated by YgfC.
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