Reconstitution of biological clock into phospholipid-coated microdroplet

In this paper, we try to establish newly technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The biological clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ^24-hour (circadian) period. Only three proteins in cyanobacteria reconstituted biological clock. We reconstitute these proteins and adenosine triphosphate (ATP) into phospholipid-coated microdroplet and confirmed the clock function. This system will reveal more detailed about biological clock system and bring application possibility, such as drug delivery.