羊脑谷胱甘肽还原酶的动力学性质。

Enzyme Pub Date : 1991-01-01 DOI:10.1159/000468877

N L Acan, E F Tezcan

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引用次数: 9

摘要

研究了羊脑谷胱甘肽还原酶(GSSGR)的动力学特性。酶具有正向双底物抑制的乒乓动力学。NADPH和GSSG的Km值分别为60.9和116.9 mumol/l, Ki值分别为42.1和347.3 mumol/l。低固定浓度NADPH对NADP+的抑制为混合型，Ki为281.5 μ mol/l， α为0.048。结果表明，该酶具有乒乓有序支链机制。

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Kinetic properties of sheep brain glutathione reductase.

The kinetic properties of sheep brain glutathione reductase (GSSGR) were investigated. The enzyme showed Ping-Pong kinetics with double substrate inhibition in the forward direction. Km values for NADPH and GSSG were found to be 60.9 and 116.9 mumol/l, and Ki values were found to be 42.1 and 347.3 mumol/l, respectively. NADP+ inhibition at low fixed concentration of NADPH was mixed-type with a Ki of 281.5 mumol/l and alpha of 0.048. It is concluded that the enzyme shows a hybrid Ping-Pong-ordered branched mechanism.

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