Cell-based field effect devices for cell adhesion analysis

We have developed cell-based field effect devices for cell adhesion analysis. Mouse fibroblast cells were adhered on the Si3 N4 gate surface of the field effect devices. Attachment and detachment of the cell could be detected as a shift of the flat hand voltage of the field effect devices based on the negative charges of sialic acid at the surface of the cell membrane. Quantitative analysis of cell adhesion could be demonstrated when diluted cell samples are used. The cell-based field effect devices are useful to study cell adhesion characteristics on different surface materials and structures as well as cell functional analysis