{"title":"腹水肿瘤原生细胞中两种折射颗粒荧光的光谱动力学特征。","authors":"P M Shvartsburd, K B Aslanidi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The spectrokinetic parameters of two different types of fluorescence of lipid-containing refractory granules (RG1 and RG2) specific to native single cells from rat ovarian tumour and Zaidela ascites hepatoma were studied. With these parameters it was possible for the first time to reveal and estimate the contribution of fluorescent lipid peroxidation products to the total fluorescence band (430-470 nm) of RG1 as well as to reveal another fluorescent component with parameters similar to those of NAD(P)H. The phenomenon of 'photoinflammation' of RG2 fluorescence under UV light, reported previously for lipofuscin granules, was observed in vitro for four retinal derivatives, but only in the absence of their contact with air.</p>","PeriodicalId":77499,"journal":{"name":"Biomedical science","volume":"2 4","pages":"391-7"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Spectrokinetic characteristics of two types of fluorescence of refractive granules in native individual cells from ascitic tumours.\",\"authors\":\"P M Shvartsburd, K B Aslanidi\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The spectrokinetic parameters of two different types of fluorescence of lipid-containing refractory granules (RG1 and RG2) specific to native single cells from rat ovarian tumour and Zaidela ascites hepatoma were studied. With these parameters it was possible for the first time to reveal and estimate the contribution of fluorescent lipid peroxidation products to the total fluorescence band (430-470 nm) of RG1 as well as to reveal another fluorescent component with parameters similar to those of NAD(P)H. The phenomenon of 'photoinflammation' of RG2 fluorescence under UV light, reported previously for lipofuscin granules, was observed in vitro for four retinal derivatives, but only in the absence of their contact with air.</p>\",\"PeriodicalId\":77499,\"journal\":{\"name\":\"Biomedical science\",\"volume\":\"2 4\",\"pages\":\"391-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1991-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical science","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Spectrokinetic characteristics of two types of fluorescence of refractive granules in native individual cells from ascitic tumours.
The spectrokinetic parameters of two different types of fluorescence of lipid-containing refractory granules (RG1 and RG2) specific to native single cells from rat ovarian tumour and Zaidela ascites hepatoma were studied. With these parameters it was possible for the first time to reveal and estimate the contribution of fluorescent lipid peroxidation products to the total fluorescence band (430-470 nm) of RG1 as well as to reveal another fluorescent component with parameters similar to those of NAD(P)H. The phenomenon of 'photoinflammation' of RG2 fluorescence under UV light, reported previously for lipofuscin granules, was observed in vitro for four retinal derivatives, but only in the absence of their contact with air.
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