NU9056 targets KAT5 to regulate the Proliferation, migration and invasion of esophageal cancer cells via ABCE1 acetylation

Objective: To explore the mechanism by which NU9056 affects the epithelial mesenchymal transformation (EMT), proliferation, migration, and invasion by regulating ABCE1 protein acetylation through KAT5. Methods: TE-1 cells were cultured in vitro, NU9056 concentration by MTT, KAT5 mRN by qRT-PCR, ABCE1 acetylation by immunoprecipitation, KAT5 and EMT-related proteins by Western blot, MTT, migration, and invasion by Transwell. Results: The optimal administration concentration of NU9056 was 1.0 µmol/L via the MTT. In the NU9056 group, KAT5 mRNA and protein expression decreased, and ABCE1 acetylation level decreased (P<0.05); in the NU9056 group, EMT marker protein E-cadherin was downregulated, while N-cadherin and Slug proteins were downregulated (P<0.05). TE-1 cell survival, migration, and invasion were significantly decreased in the NU9056 group (P<0.05). Conclusion: NU9056 may reduce the ABCE1 protein acetylation levels by downregulating KAT5 expression, and subsequently inhibit the EMT, survival, migration, and invasion capacity of esophageal cancer cells. Key words: Acetyltransferase inhibitor NU9056 KAT5 ABCE1, modified by acetylation