向日葵蜂蜜在实验室条件下对从有消化不良迹象的蜜蜂中分离出来的混合微生物协会的活性研究

T. Romanishina, T. Kot, S. Guralska, S. Furman, Z. Rybachuk, O. Kysterna
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引用次数: 0

摘要

养蜂业是乌克兰农业的重要部门之一,其主要活动是培育蜜蜂,获得蜂蜜和其他有益人类健康的产品。保持蜂群的生理状态在适当的水平是兽医学医生和养蜂人的基本任务之一。在养蜂场遵守兽医和卫生要求是预防蜂群传染性疾病的基础。由于昆虫的身体有一个快速的新陈代谢,任何传染性病理很快获得大量的表现。蜜蜂“肠道”感染性损伤的一个指示性症状是消化不良。在这种情况下,蜜蜂会出现腹泻、厌食和生产力下降。在养蜂中预防此类病理表现的手段市场相对狭窄。自古以来,人们就知道蜂蜜的治疗和有益特性,表现在止痛、抗菌和抗炎的作用上。向日葵蜂蜜与其他类型蜂蜜的不同之处在于其成分的范围很广,尤其是植物杀菌剂,它能抑制病原微生物的生长。因此,本实验的主要目的是在实验室研究不同浓度的葵花蜜筛对消化不良症状蜜蜂分离的混合微生物关联的影响。采用迪斯科-扩散法,在MPA培养基(肉蛋白胨琼脂)上的培养皿中研究了向日葵蜂蜜在实验室条件下的活性。将葵花蜜用蒸馏水按1:1的比例稀释,将葵花蜜糖浆按1:2、1:4、1:10、1:100的稀释比例配制成工作溶液。数据分析表明,在所有研究浓度下,向日葵蜂蜜糖浆的抑菌活性都与混合微生物关联有关。此外,当向日葵蜂蜜糖浆以1:10(19.2±0.42 mm)的比例稀释时,所研究的微生物的生长抑制带直径最大。结果表明,向日葵蜂蜜糖浆对混合微生物的拮抗作用在不同的稀释度下均有明显的增强,其中以天然工作液浸渍的圆盘拮抗直径最大,为19.6±0.27 mm。因此,积累和鉴定一种纯培养拮抗剂是有希望的,它涉及蜜蜂腹泻期间分离的细菌的混合培养,从而进一步创造一种预防不健康蜂房中蜜蜂感染病理的药理学剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Study of the activity of sunflower honey against a mixed microbial association isolated from bees with signs of dyspepsia in laboratory conditions
Beekeeping is one of the essential branches of agriculture in Ukraine, the main activity of which is aimed at breeding bees and obtaining honey and other products beneficial for human health. Maintaining the physiological state of bee colonies at the proper level is one of the essential tasks of veterinary medicine doctors and beekeepers. Compliance with veterinary and sanitary requirements at bee farms is the basis of preventing infectious pathologies in bee colonies. Since the body of insects has a fast metabolism, any infectious pathology quickly acquires a massive manifestation. One indicative symptom of infectious damage to the “gut” of bees is dyspepsia. Diarrhea, anorexia, and loss of productivity are observed in bees in this condition. The market of means to prevent such pathological manifestations in beekeeping is relatively narrow. The healing and favorable properties of honey, manifested in pain-relieving, antimicrobial and anti-inflammatory effects, have been known since ancient times. Sunflower honey differs from other types in a wide range of components, particularly phytoncides, which inhibit the growth of pathogenic microorganisms. Therefore, the main goal of the experiment was a laboratory study of the effect of different concentrations of sunflower honey sieve on the mixed microbial association isolated from bees with signs of dyspepsia. The activity of sunflower honey in laboratory conditions was studied by the disco-diffusion method in Petri dishes on MPA medium (meat-peptone agar). To obtain syrup from sunflower honey, sunflower honey was diluted with distilled water in a ratio of 1:1, working solutions were prepared from sunflower honey syrup in dilutions of 1:2, 1:4, 1:10, 1:100. The data analysis indicates the bacteriostatic activity of the syrup from sunflower honey in all studied concentrations concerning the mixed microbial association. Moreover, the largest diameter of the growth inhibition zone of the studied microorganisms was noted when the syrup from sunflower honey was diluted in a ratio of 1:10 (19.2 ± 0.42 mm). In turn, the antagonistic effect of the syrup from sunflower honey was observed in all dilutions concerning the mixed microbial association, where the largest diameter of the antagonism was 19.6 ± 0.27 mm when the disc was impregnated with the native working solution. Thus, it is promising to accumulate and identify a pure culture antagonist concerning a mixed culture of bacteria isolated during bee diarrhea to further create a pharmacological agent for preventing infectious pathologies of bees in unhealthy apiaries.
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