库尔德斯坦草莓品种γ辐照诱变、变异产生及适宜辐照剂量的确定

H. Razmi, Reza Amiri Fahliani, B. Kavoosi, A. M. Asl, Fars Agric. Hort. Crops Res., N. Res.
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摘要

由于草莓的无性繁殖,其遗传多样性较低。增加遗传多样性的方法之一是利用伽马射线诱导突变,这是植物育种的重要手段。为了确定库尔德斯坦草莓品种突变创造遗传多样性的适宜辐照剂量,研究不同辐照剂量(0(对照)、15、30、60和90灰度)对草莓不同性状的影响,本研究于2012-2013年在Yasouj大学农学院进行。结果表明,除叶片数、果糖和花青素外,辐照植株在大部分指标上均低于对照植株。随着辐照剂量的增加,叶片面积、果实长度、果实直径、果实鲜重和干重以及果实体积、形状和汁液均呈下降趋势。此外,辐照植株的多叶化和矮化现象增加。在库尔德斯坦草莓品种中,γ辐照诱变的适宜剂量约为65-70灰色,处理后植株死亡率阈值为50%。因此,在草莓果实品质改良等育种项目中,在推荐限度内的伽马射线强度可用于创造最高的遗传多样性和最低的植株死亡率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mutation Induction and Creation of Variation in Strawberry, Kurdistan Cultivar, by Gamma Irradiation and Determination of Suitable Irradiation Dosage
Genetic diversity in strawberry is low due to its asexual propagation. One method of increasing genetic diversity, as an important tool in plant breeding, is the use of gamma rays to induce mutation. In order to determine the appropriate dosage of gamma irradiation for genetic diversity creation by mutation in strawberry, Kurdistan cultivar, and to study the influence of various doses of gamma ray (zero (control), 15, 30, 60 and 90 gray) on different traits of strawbery, this research was conducted in Faculty of Agriculture, Yasouj University, in 2012-2013. Results showed that irradiated plants had a lower rates in comparison to the control plants for most of the studied characteristics, except for number of leaves, fruit sugar and anthocyanin. Leaf area, fruit length, fruit diameter, fruit fresh and dried weight, and fruit volume, shape, and juice decreased with an increase in the gamma irradiation dosage. Moreover, multifoliate and dwarfism were increased in the irradiated plants. The appropriate dosage of gamma irradiation for mutation induction in strawberry, Kurdistan cultivar, for threshold 50% plants mortality (LD 50 ) treated by gamma ray, was approximately 65-70 gray. Therefore, gamma ray intensity in the recommended limit could be used for the highest genetic diversity creation with the lowest plant mortality in strawberry breeding programs such as in fruit quality improvement.
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