Extremophiles Rescue from -80°C Freeze-stocked Deep-sea Cores and Metagenomic Analysis of Living Microorganisms

Kochi core center (KCC) is one of four International Ocean Discovery Program (IODP) curation centers located around the world. At IODP curation centers, a lot of core samples are stored in deep freezer rooms and are provided to researchers worldwide. These are important resources for researching novel ecosystem communities, earthquake mechanisms, and methane hydrate deposits. Ideally, researchers hope to use fresh cores immediately after collection on ship. On the other hand, we know a lot of important core samples are frozen at several institute centers and we should make good use of them. In this experiment, we tried to extract the bacterial community from long-term stored core samples using an attached potentialcontrolled electrode. This result shows that approximately 106 cells/g Research Article