Characterization of cell protection effects of Twendee X by oxidative stress.

[Purposes] Reactive Oxygen Species (ROS) damages cell and mitochondria (MT) as well. Twendee X (TWX) is a composition consisting of Vitamin C, Glutamine, Cystine or Cysteine, Riboflavin, Succinic acid, Fumaric acid, Coenzyme Q10, and Niacin. TWX strongly reduces ROS (Patent: WIPO WO2013/072441 A1, COMPOSITION FOR PROTECTION AGAINST CELL-DAMAGING EFFECTS). Ant-ROS and cell protection effects of TWX in vivo and vitro were examined. [Experiment] Amount of TWX administrated to mouse was 15-20mg/kg. Mouse: C57BL, male. Parameters of ROS were d-ROMs test (hydrogen peroxide level), BAP test (Biological Antioxidant Potential) and OXY Adsorbent test (Elimination ability of the hypochlorous acid). Cell culture experiments using HepG2 cell line was conducted by ICDD (France). [Data] Administration of TWX to radiation mouse model 3 days and 7days reduced d-ROMs test 65% and 111% respectively. Survival of mouse radiation was significantly prolonged by Twendee X administration. ICDD reported that TWX reduces ROS of cell: 45% and MT: 63%, increase Superoxide Dismutase of cell: 60% and MT: 147%. [Conclusion] ICDD confirmed that TWX is most strong anti-ROS and protection effects from ROS. TWX is a very safe composition for human, and already started human clinical study.