Biomolecular detection with an interferometric microfiber-capillary optofluidic sensor

We have developed a chip-scale optofluidic sensor for biomolecular detection, by tapering laterally aligned silica microfiber and capillary to form a modal interferometer. With the pre-immobilization of DNA probes, the sensor is capable of selectively detecting single-stranded microRNA-let7a (molecular weight: 6.5 k) by measuring the spectral shift of the interferometric spectrum. A log-linear response from 2 nM to 20 μΜ and a minimum detectable concentration of 212 pM (1.43 ng/mL) have been achieved. The sensor is promising for future diagnosis applications due to its high sensitivity, resistance to environmental perturbations, improved portability, and intrinsic connection to fiber optic measurement.