{"title":"伊拉克AL-QADISIYAH省牛隐孢子虫分离株基因型分析","authors":"ِِAsmaa Al-Jawasim","doi":"10.23975/bjvetr.2022.172808","DOIUrl":null,"url":null,"abstract":"The current research included examination of 100 fecal sample from bovine was collected from AL-Qadisiyah province, from September 2018 until February 2019.The Microscopically result showed that oval or spherical shaped with dark pink color or red oocyst on blue ground and 30(30%) positive sample out 100 case . It was recorded that the maximum rate 41.66% (5/12) was seen in November, but the lowest rate 18.75% (3/16) was seen in the month of February with no significant differences at level (p<0.05.) According to age the maximum rate of incidence 40%(14/35)was found in the age group lower than a month, but the lowest incidence was seen in the group( >1 years). There is no significant differences at p<0.05.between male and female. In the currently study the N-PCR in molecular investigation were used ,the positive sample was18 (60%) out of 30 fecal sample. Sequencing of a fragment of the(18s rRNA) gene (834 bp) that separated from many distinct area in AL- Qadisiyah government recorded (50%)6/12 sample related to NCBI – Blast Cryptosporidium parvum ,( 33.33%) 4/12 sample display deep related to NCBI –Blast Cryptosporidium bovis ( this first study reported C. bovis in Iraq ) , (16.66%) 2/12 sample showed closed related to NCBI –Blast Cryptosporidiumandersoni.","PeriodicalId":218727,"journal":{"name":"Basrah Journal of Veterinary Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"GENOTYPE OF Cryptosporidium spp. ISOLATED FROM BOVINE OF AL-QADISIYAH PROVINCE /IRAQ\",\"authors\":\"ِِAsmaa Al-Jawasim\",\"doi\":\"10.23975/bjvetr.2022.172808\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The current research included examination of 100 fecal sample from bovine was collected from AL-Qadisiyah province, from September 2018 until February 2019.The Microscopically result showed that oval or spherical shaped with dark pink color or red oocyst on blue ground and 30(30%) positive sample out 100 case . It was recorded that the maximum rate 41.66% (5/12) was seen in November, but the lowest rate 18.75% (3/16) was seen in the month of February with no significant differences at level (p<0.05.) According to age the maximum rate of incidence 40%(14/35)was found in the age group lower than a month, but the lowest incidence was seen in the group( >1 years). There is no significant differences at p<0.05.between male and female. In the currently study the N-PCR in molecular investigation were used ,the positive sample was18 (60%) out of 30 fecal sample. Sequencing of a fragment of the(18s rRNA) gene (834 bp) that separated from many distinct area in AL- Qadisiyah government recorded (50%)6/12 sample related to NCBI – Blast Cryptosporidium parvum ,( 33.33%) 4/12 sample display deep related to NCBI –Blast Cryptosporidium bovis ( this first study reported C. bovis in Iraq ) , (16.66%) 2/12 sample showed closed related to NCBI –Blast Cryptosporidiumandersoni.\",\"PeriodicalId\":218727,\"journal\":{\"name\":\"Basrah Journal of Veterinary Research\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-02-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Basrah Journal of Veterinary Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.23975/bjvetr.2022.172808\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Basrah Journal of Veterinary Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.23975/bjvetr.2022.172808","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
GENOTYPE OF Cryptosporidium spp. ISOLATED FROM BOVINE OF AL-QADISIYAH PROVINCE /IRAQ
The current research included examination of 100 fecal sample from bovine was collected from AL-Qadisiyah province, from September 2018 until February 2019.The Microscopically result showed that oval or spherical shaped with dark pink color or red oocyst on blue ground and 30(30%) positive sample out 100 case . It was recorded that the maximum rate 41.66% (5/12) was seen in November, but the lowest rate 18.75% (3/16) was seen in the month of February with no significant differences at level (p<0.05.) According to age the maximum rate of incidence 40%(14/35)was found in the age group lower than a month, but the lowest incidence was seen in the group( >1 years). There is no significant differences at p<0.05.between male and female. In the currently study the N-PCR in molecular investigation were used ,the positive sample was18 (60%) out of 30 fecal sample. Sequencing of a fragment of the(18s rRNA) gene (834 bp) that separated from many distinct area in AL- Qadisiyah government recorded (50%)6/12 sample related to NCBI – Blast Cryptosporidium parvum ,( 33.33%) 4/12 sample display deep related to NCBI –Blast Cryptosporidium bovis ( this first study reported C. bovis in Iraq ) , (16.66%) 2/12 sample showed closed related to NCBI –Blast Cryptosporidiumandersoni.