制定有效的疫苗接种方案以生产无沙门氏菌的蛋鸡

T. Akter, M. Nooruzzaman, T. T. Mumu, M. Ahammed, A. Uddin, R. Parvin, M. A. H. N. A. Khan, M. Hossain
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引用次数: 2

摘要

沙门氏菌感染是蛋鸡行业的头等大事,对公众健康构成重大威胁。出口优质家禽产品的生产需要无沙门氏菌认证。我们在鸡群中开发并应用了一种基于商业减毒肠沙门氏菌血清型鸡种生物变异鸡种(S. Gallinarum)疫苗的疫苗接种计划。1000只ISA褐鸡每隔一段时间接种冻干禽伤寒活疫苗。采用血清平板凝集法(SPA)和酶联免疫吸附试验(ELISA)检测小鼠粪拭子中沙门菌的血清抗体反应。在接种疫苗时(16周龄),50%的受试禽类粪便中携带沙门氏菌。在18 w、21 w和每隔12周加强免疫后,沙门氏菌的脱落率随时间的推移显著下降,21 w时为40%,30 w时为10%,42 w和54 w时为5%,66 w和78 w时未检测到脱落。对32株沙门氏菌进行生化分析,发现15.6% (n=5)的肠道沙门氏菌为血清型鸡痢菌(S. Pullorum), 37.5% (n=12)的鸡痢沙门氏菌(S. Pullorum), 46.9% (n=15)的副伤寒沙门氏菌引起。值得注意的是,21 w后检测到的沙门氏菌属副伤寒组。细菌的减少与抗体反应的增加是平行的。血清平板凝集(SPA)试验显示,接种疫苗时抗体水平(16w时阳性率为20%)随时间增加,18w时阳性率为50%,42w时阳性率为80%,54w时阳性率为90%,66w和78w时阳性率为100%。同样,采用ELISA法测定鸡的血清抗体水平,与SPA试验相似。总之,本研究开发的疫苗计划证实了高血清转化,并防止沙门氏菌在粪便中脱落。因此,建议在产蛋前期至产蛋后期接种3个月间隔疫苗,以预防产蛋群中的沙门氏菌感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of an effective vaccination protocol to produce Salmonella-free layer fock
Salmonella infections are of prime importance in the layer chicken industry and pose a significant public health threat. Production of export quality poultry products requires Salmonella-free certification. We developed and applied a vaccination schedule based on a commercial live attenuated Salmonella enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) vaccine in a layer flock. A flock of 1000 ISA Brown chickens has vaccinated with a lyophilized fowl typhoid live vaccine at definite time intervals. Isolation of Salmonella spp. in cloacal swabs and detection of serum antibody responses were performed using serum plate agglutination (SPA) test and ELISA. At the time of vaccination (16 weeks (w) of age), 50% of the tested birds carried Salmonella spp. in feces. Following booster immunization at 18 w, 21 w, and then every 12 weeks interval, the shedding of Salmonella decreased significantly over time with 40% at 21 w, 10% at 30 w, and 5% each at 42 and 54 w, and no shedding was detected at 66 and 78 w. Biochemical analysis of 32 Salmonella isolates revealed 15.6% (n=5) Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum), 37.5% (n=12) Salmonella Gallinarum, and 46.9% (n=15) paratyphoid causing Salmonella. Of note, the Salmonella spp. detected after 21 w of age belonged to the paratyphoid group. The decreased shedding of bacteria paralleled with increased antibody responses. The antibody level at vaccination (20% positive rate at 16 w) increased over time with a 50% positive rate at 18 w, 80% at 42 w, 90% at 54 w, and 100% at 66 w and 78 w by serum plate agglutination (SPA) test. Similarly, the serum antibody levels of chickens were also measured using ELISA and were similar to the SPA test. In conclusion, the vaccine schedule developed in this study confirmed a high seroconversion and prevented Salmonella shedding in feces. Therefore, a three-month interval vaccination protocol from the pre-laying stage to the last stage of laying is recommended to prevent Salmonella infections in laying flocks.
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