猕猴皮层深层三光子功能成像的研究进展

Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN) Pub Date : 1900-01-01 DOI:10.1364/brain.2023.btu1b.6

K. Takasaki, Gaku Hatanaka, Soumya Chatterjee, P. Balaram, Taekjun Kim, Celeste Dylla, Farzaneh Abolmaali, R. Reid, Anitha Pasupathy, W. Bair, Jack Waters

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引用次数: 0

摘要

3光子激发激光扫描显微镜使荧光成像深入组织超出2光子显微镜的范围。在这里，我们报告了非人类灵长类动物神经元功能钙成像的3光子显微镜的结果。我们比较了2光子和3光子激发，并在超过2光子极限的深度下表征了3光子显微镜的性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Development of 3-Photon Functional Imaging in Deep Layers of Macaque Cortex

3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.

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Biophotonics Congress: Optics in the Life Sciences 2023 (OMA, NTM, BODA, OMP, BRAIN)

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