不同硼盐处理对东方野檀离体培养的微繁殖和遗传稳定性的影响

Taner Mercan, S. Galatali, Damla Ekin ]Özkaya, O. Çelįk, E. Kaya
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引用次数: 1

摘要

采用ISSR标记技术,研究了硼盐对残地植物Liquidambar orientalis微繁殖的影响,并测定了其微芽的遗传稳定性。特别是在低盐耐旱物种中,盐胁迫可能引起植物生长发育、次生代谢物含量响应胁迫增加、体细胞无性系变异等生理和分子变化。采用硼酸盐、过硼酸钠盐、偏硼酸钠盐和辛酸二钠盐3种不同浓度的硼酸盐处理离体克隆繁殖的东方白羊草分生组织,研究了硼盐对分生组织再生和发育的影响。与不施盐的对照组样品相比,再生率最佳的处理为1mg -1辛酸二钠处理,其值为100%,而在评价芽形成能力指数时,5mg -1过硼酸钠处理,其值为4.94,效果最佳。然而,与母本相比,所有盐处理均引起遗传稳定性的体细胞无性系变异,根据分析的数据,所有盐处理均确定了最低30% (5mg -1辛酸二钠)和最高49% (1 mg -1硼酸)的体细胞无性系变异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of different boron salt treatments on micropropagation and genetic stability in in vitro cultures of Liquidambar orientalis Miller
In the present study, the boron salts effects on the micropropagation of Liquidambar orientalis, a relict-endemic plant species, were investigated and genetic stability of micro-shoots was determined by ISSR marker technique. Especially in species with low salinity and drought tolerance, salt stress may cause physiological and molecular changes such as plant growth and development, increase in secondary metabolite content in response to stress, and somaclonal variation. In this context, three different concentrations of boric acid, sodium perborate, sodium metaborate and disodium octaborate salts were applied to meristems isolated from in vitro clonal propagated L. orientalis and the effects of these boron salts on meristem regeneration and development were evaluated. When compared to the control group samples in which no salt application was applied, the best regeneration percentage was determined as 1mgL-1 disodium octaborate treatment with a value of 100%, while when the shoot forming capacity index was evaluated, 5mgL-1 sodium perborate treatment with a value of 4.94 gave the best results. However, when compared with the mother plant, it was observed that all salt treatments caused somaclonal variation on genetic stability, and in the light of the analyzed data, the lowest 30% (5mgL-1 disodium octaborate) and the highest 49% (1 mgL-1 boric acid) somaclonal variation were determined in all applications.
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