Ado Ekiti中央屠宰场屠宰板的微生物评估

E. O. Arire, A. Sulaimon, C. Samuel
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摘要

这项研究的目的是评估其他国家选定的屠宰板中微生物的存在,以告知动物科学家、公共卫生工作者、屠夫和肉类供应商了解受污染肉类对人类健康构成的风险。在Ado-Ekiti中心屠宰场,共使用50根无菌拭子棒从5个屠宰场屠宰板表面收集样本,每个屠宰板收集10个拭子样本。样品收集后立即送往实验室进行微生物学研究。采用胰酶-大豆琼脂(TSA)培养基,氯霉素替代营养细菌培养基,沙伯劳-葡萄糖-琼脂(SGA)替代真菌培养基。培养皿37℃孵育48 - 72小时,每天使用立体显微镜观察培养物是否存在真菌菌丝和菌落。结果显示,细菌计数平均值为1.83 × 105 CFU/ml,真菌计数平均值为0.59 × 105 CFU/ml。从所有样本中分离出革兰氏阳性菌和革兰氏阴性菌共8株,其中金黄色葡萄球菌的发生率最高(33%),其次是大肠杆菌(26%)、铜绿假单胞菌(19%),其他流行菌各占5%。在真菌样品中,分离出4株真菌,在所有真菌总数中,黄曲霉(Aspergillus flavus)的发生率最高,约为40%,青霉菌(Penicillium spp)的发生率为28%,真菌总数最低的为8%。研究表明,屠宰板表面的肉具有较高的污染潜力。结果表明,污染率可归因于卫生水平差,在Ado Ekiti的中央屠宰场没有采取适当措施和注意卫生习惯。这就要求对屠夫进行教育,让他们了解保持良好卫生习惯的重要性,用现代技术处理肉类,并鼓励在屠宰场保持良好的卫生条件。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial Assessment of Slaughter Slabs at the Central Slaughterhouse of Ado Ekiti
This study was aimed at assessing the presence of microbes in the selected slaughter slabs in other to inform the Animal scientists, Public health workers, Butchers and Meat vendors to know the risk pose to human health with contaminated meat. A total of 50 sterile swab sticks were used to collect the samples from five abattoir slaughter slabs surfaces in Ado-Ekiti central abattoir with ten swab samples from each slaughter slab. After collection the samples were immediately transported to laboratory for microbiological studies. Trypticase-soya-agar (TSA) was used as medium for the samples, while chloramphenicol was used in replacement to trophic bacteria and Sabouraud-glucosea-gar (SGA) for fungi. The incubation of Petri dishes were set at 37ºC for 48 – 72hrs while daily observation of the cultures were carried out with the use of stereoscopic microscope for the presence of fungal mycelium and or bacterial colonies. The results revealed that bacterial count mean value was 1.83 × 105 CFU/ml and fungal count mean value was 0.59 × 105 CFU/ml. A total of eight strains of bacteria comprises of gram positive and gram negative bacteria from all the samples were isolated, Staphylococcus aureus had the highest percentage of occurrences (33%), followed by Escherichia coli (26%), Pseudomonas aeruginosa (19%), while other prevalence bacteria have 5% each. In the fungi samples, isolation of four strains were carried out, from all the total fungal count, Aspergillus flavus had the highest number of occurrence with approximately 40%, Penicillium spp had 28%, and 8% occurrence for the lowest fungal count. It is shown in the study that the meat has higher contamination potential from the slaughter slab surfaces. With the result shown, contamination rate could be attributed to poor sanitation level, and proper measure and attention was not given to hygienic practices in the Central abattoir in Ado Ekiti. This then called for butchers education on the importance of practicing good hygiene, handling of meat with modern techniques and encouragement of good sanitation in the abattoirs.
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