【用染色剂检测大肠杆菌和沙门氏菌的致病因素】。

Laboratornoe delo Pub Date : 1991-01-01
L V Grigor'eva, G I Korchak, L A Malakhova
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引用次数: 0

摘要

作者改进了使用三种染色剂检测肠杆菌致病性因子的技术。该方法已在306株大肠杆菌和沙门氏菌中进行了试验。在培养物生长表面分层蓝色伊红和在固体培养基中加入刚果红时,得到最准确的结果。结晶紫分层产生的结果不太具体。上面列出的技术帮助检测了高达25- 30%的埃希氏菌和高达17%的沙门氏菌菌株的致病性因子。建议在实际研究中与其他试验方法结合使用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[The use of stains for the detection of the pathogenetic factors of Escherichia and Salmonella].

The authors have modified the technique for using three stains to detect enterobacterial pathogenicity factors. The methods have been tried with 306 Escherichia and Salmonella strains. The most accurate results were obtained when azure eosin was layered onto culture growth surface and when Congo red was added into solid medium. Crystal violet layering yielded less specific results. The techniques listed above helped detect pathogenicity factors of up to 25-30 percent of Escherichia and up to 17 percent of Salmonella strains. The methods are recommended to be used in complex with other tests in practical studies.

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