控制dna -鱼精蛋白复合物的高阶结构

Y. Yoshikawa, N. Makita, Mari Suzuki, Eri Shindo, Chika Watanabe, T. Kanai, T. Imanaka, T. Kanbe, K. Yoshikawa
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引用次数: 0

摘要

我们利用荧光显微镜和电子显微镜研究了大DNA与小碱性蛋白鱼精蛋白的相互作用。在大多数脊椎动物的精子细胞中,一个大的DNA被鱼精蛋白包装成一个高度紧密的,生物无活性的染色质形式。包装方式在精子发生中起着关键作用。我们发现,随着鱼精蛋白浓度的增加,DNA分子通过中间态的发生,呈现出由细长线圈态向致密态的构象变化。我们还证明了DNA-鱼精蛋白复合物的高阶结构可以用光镊(Nd YAG激光,1064 nm)控制,而不需要对DNA进行任何结构修饰,如塑料珠的附着。这些结果在生物学意义上进行了讨论。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Controlling the higher-order structure of DNA-protamine complex
We investigated the interaction of large DNA with protamine, a small basic protein, using fluorescence microscopy and electron microscopy. In most vertebrate sperm cells, a large DNA is packaged by protamine into a highly compact, biologically inactive form of chromatin. The manner of packaging plays a key role in spermatogenesis. We found that, with increase of protamine concentration, DNA molecules show the conformational change from an elongated coil state into a compact state through the occurrence of the intermediate state. We also show that the higher-order structure of DNA-protamine complex can be controlled by optical tweezers (Nd YAG laser, 1064 nm) without any structural modification of DNA such as an attachment of plastic beads. These results are discussed in relation to the biological meaning.
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