{"title":"循环肿瘤细胞捕获微流体装置中蛋白质固定的优化","authors":"M. Gaskill, C. Launiere, D. Eddington","doi":"10.5210/JUR.V5I1.7504","DOIUrl":null,"url":null,"abstract":"The isolation of metastatic circulating tumor cells (CTCs) within the blood using microfluidic devices is a promising method for the detection of cancer. In this study, protein patterning of endothelial-leukocyte adhesion molecule-1 (E-selectin) and anti-epitheal-cell-adhesion-molecule (Anti-EpCAM) within microfluidic channels is utilized to improve the capture efficiency. To create this protein patterning the protein immobilization method must first be optimized to allow for maximum capture of CTCs and minimal increase in the flow rate of cells through the device due to added coating thickness. Proteins are immobilized in alternating regions using photo-initiated graft polymerization of polyacrylic acid (PAA) and a silanization reaction. Using interferometer measurements and fluorescent tagging, PAA height was minimized and protein immobilization was optimized.","PeriodicalId":426348,"journal":{"name":"The Journal of Undergraduate Research at the University of Illinois at Chicago","volume":"56 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Optimization of Protein Immobilization in Microfludic Devices for Circulating Tumor Cell Capture\",\"authors\":\"M. Gaskill, C. Launiere, D. Eddington\",\"doi\":\"10.5210/JUR.V5I1.7504\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The isolation of metastatic circulating tumor cells (CTCs) within the blood using microfluidic devices is a promising method for the detection of cancer. In this study, protein patterning of endothelial-leukocyte adhesion molecule-1 (E-selectin) and anti-epitheal-cell-adhesion-molecule (Anti-EpCAM) within microfluidic channels is utilized to improve the capture efficiency. To create this protein patterning the protein immobilization method must first be optimized to allow for maximum capture of CTCs and minimal increase in the flow rate of cells through the device due to added coating thickness. Proteins are immobilized in alternating regions using photo-initiated graft polymerization of polyacrylic acid (PAA) and a silanization reaction. Using interferometer measurements and fluorescent tagging, PAA height was minimized and protein immobilization was optimized.\",\"PeriodicalId\":426348,\"journal\":{\"name\":\"The Journal of Undergraduate Research at the University of Illinois at Chicago\",\"volume\":\"56 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-03-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Undergraduate Research at the University of Illinois at Chicago\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5210/JUR.V5I1.7504\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Undergraduate Research at the University of Illinois at Chicago","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5210/JUR.V5I1.7504","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Optimization of Protein Immobilization in Microfludic Devices for Circulating Tumor Cell Capture
The isolation of metastatic circulating tumor cells (CTCs) within the blood using microfluidic devices is a promising method for the detection of cancer. In this study, protein patterning of endothelial-leukocyte adhesion molecule-1 (E-selectin) and anti-epitheal-cell-adhesion-molecule (Anti-EpCAM) within microfluidic channels is utilized to improve the capture efficiency. To create this protein patterning the protein immobilization method must first be optimized to allow for maximum capture of CTCs and minimal increase in the flow rate of cells through the device due to added coating thickness. Proteins are immobilized in alternating regions using photo-initiated graft polymerization of polyacrylic acid (PAA) and a silanization reaction. Using interferometer measurements and fluorescent tagging, PAA height was minimized and protein immobilization was optimized.
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