孟加拉国几种小型乡土水果的生物活性成分、生化和抗氧化特性测定

Mohammad Mainuddin Molla, Ashfak Ahmed Sabuz, Md. Golam Ferdous Chowdhury, Md. Hafizul Haque Khan, M. Alam, T. Nasrin, A. Khatun, M. M. Islam
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引用次数: 0

摘要

少量水果可能含有抗营养素,但在孟加拉国没有全面的原始数据。因此,目前的研究是为了收集次要水果成分数据库的数据而进行的。采用1,1-二苯基-2苦味酰肼(DPPH)清除率和还原力测定法评价了所选小果(RPA)的总酚(TPH)、维生素C、总胡萝卜素和s-胡萝卜素含量及其抗氧化活性。采用光电二极管阵列检测器和自动进样器的高效液相色谱法分析酚类化合物。结果表明,小果实含有多种植物化学物质,特别是TPH、抗坏血酸、总黄酮、s-胡萝卜素、总类胡萝卜素和总花青素含量;分别为0.23 ~ 176.50 mg GAE/g、16.67 ~ 664.92 mg/100 g、2.26 ~ 150.02 mg QE/100 g、1.41 ~ 6897.57µg/100 g、1.26 ~ 98.24 mg/100 g和1.15 ~ 47.46 mg/100 g。在抗氧化能力、总抗氧化能力、DPPH、还原能力(RPC)、铁还原能力(FRAP)、金属螯合能力(MCC)、一氧化氮(NO)和自由基清除能力等参数中,抗坏血酸/mg提取物的IC50分别为0.01 ~ 278.24µg、39.70 ~ 250.00%、3.21 ~ 634.00%、0.02 ~ 1817.88µM Fe2SO4/100g、22.29 ~ 210.43%、0.02 ~ 70.50%和4.98 ~ 856.70µg/g。已发现并定量的酚酸主要有没食子酸(279.06 mg/100 g)、香草酸(43.77 mg/100 g)、Þ-courmaric酸(178.96 mg/100 g)、阿魏酸(20.44 mg/100 g)和叶黄素(91.13 μ g/100 g),分别存在于黄芪、白天果、象苹果和枸杞中。此外,所有的小水果都富含生物活性,生化和抗氧化化合物,可用于治疗应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of Bioactive Compounds, Biochemical and Antioxidant Properties of Selected Minor Indigenous Fruits in Bangladesh
Minor fruits may contain antinutrients, but there is no comprehensive primary data available in Bangladesh. As a result, the current research was carried out in order to collect data for a database of minor fruit composition. The antioxidant activity of 1,1-diphenyl-2picryl hydrazyl (DPPH) scavenging and reducing power assays were used to evaluate the total phenolic (TPH), vitamin C, total carotene, and s-carotene contents and antioxidant activity of selected minor fruits (RPA). High-performance liquid chromatography with a photodiode array detector and autosampler was used to analyse phenolic compounds. The findings revealed that minor fruits contain a variety of phytochemicals,, particularly TPH, ascorbic acid, total flavonoid (TF), s-carotene, total carotenoid (TC), and total anthocyanin content (TAC); values ranged, respectively, 0.23-176.50 mg GAE/g, 16.67-664.92 mg/100 g, 2.26-150.02 mg QE/100 g, 1.41-6897.57 µg/100 g, 1.26-98.24 mg/100 g and 1.15-47.46 mg/100 g. In the parameters antioxidant activity, total antioxidant capacity, DPPH, reducing power capacity (RPC), ferric reducing antioxidant power (FRAP), metal chelating capacity (MCC), nitric oxide (NO), and free radical scavenging activity, IC50 ranged 0.01-278.24 µg of ascorbic acid/mg of extract, 39.70-250.00%, 3.21-634.00%, 0.02-1817.88 µM Fe2SO4/100g, 22.29-210.43%, 0.02-70.50%, and 4.98-856.70 µg/g, respectively. Phenolic acids that have been found and quantified, leading examples were gallic acid (279.06 mg/100 g), vanilic acid (43.77 mg/100 g), Þ-courmaric acid (178.96 mg/100 g), ferulic acid (20.44 mg/100 g), and lutein (91.13 µg/100 g) in aonla, day fruit, elephant apple, and bilimbi. Furthermore, all of the minor fruits are high in bioactive, biochemical, and antioxidant compounds that could be used in therapeutic applications.
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