Bioseparation of phytochemical constituents from leaf and stem extracts of Mimosa pudica L

The isolation procedure of chemical constituents was mainly based on fractionation by solvents of varying polarity. After cold extraction, solvent-solvent partitioning of extract was done with different solvents to yield different extracts of leaf and stem of Mimosa pudica L. Gas Chromatography-Mass Spectrometry (GC-MS) analysis was carried out for petroleum ether, chloroform and ethyl acetate extracts of leaf as well as chloroform and ethyl acetate extracts of stem and 16, 11, 3, 26 and 3 compounds were identified respectively. The major compounds were benzene,1-ethyl-3-methyl- (14.83%) for petroleum ether extract of leaf, fumaric acid, ethyl 2-methylallyl ester (16.96%) for chloroform extract of leaf, glycerin (74.71%) for ethyl acetate extract of leaf, ticlopidine (80.90%) for chloroform extract of stem and hexadecenoic acid, methyl ester (69.95%) for ethyl acetate extract of stem.