Bioremediasi air asam tambang batubara dengan pengayaan bakteri pereduksi sulfat dan penambahan substrat organik

Acid mine drainage (AMD) has a negative impact that must be managed because it can cause environmental pollution and disrupt mining operations. Acid mine drainage contains dissolved heavy metals that are harmful to the ecosystem. Sulfate-reducing bacteria (SRB) can be used in acid mine drainage bioremediation. This study aims to analyze pH, C-organic and BPS growing in acid water sediments of coal mines and several other ecosystems, analyze C/N ratio and pH of potential organic substrates, and recover AMD by enrichment of indigenous SRB and addition of organic substrates. The ecosystems observed were AMD sediment, paddy field mud, mangrove sediment, in situ sediment, ditch sediment, and rhizosphere soil. The organic materials tested included water hyacinth compost, a mixture of paddy field mud with wood sawdust, oil palm empty fruit bunch (EFB) compost, chicken manure, cow manure, dairy factory waste, and mixtures thereof. The results showed that SRB could be found in all studied ecosystems in the range of 5.8E+2 – 1.26E+5 MPN-unit/dry weight. The value of the C/N ratio with variations from 9.23-89.5 had a negative correlation with increasing pH. The application of organic matter with concentrations of 3%, 5%, 7%, and 10% helped SRB to raise the pH of AMD to more than 6 and reduced Fe by 88.18 – 97.27%. The highest SRB population was obtained in the mixed treatment of EFB and chicken manure with a population of 2.41E+12 MPN- unit/dry weight.