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Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) patients with lower urinary tract symptoms (LUTS) and erectile dysfunction (ED) eventually show increased sympathetic tension. Therefore, this study aimed to assess the effects of serum Immunoglobulin G (IgG) on secreted norepinephrine (NE) amounts and norepinephrine transporter (NET) expression in sympathetic neurons. The purified serum IgG from the patient group Control, CP/CPPS+ED, CP/CPPS and ED was administered to cultured superior cervical sympathetic ganglions from nude mice, and secreted NE was quantitated by radioimmunoassay. Quantitative real-time PCR and Western blot were performed to assess NET expression at the mRNA and protein levels, respectively. There was no significant difference in secreted NE levels among the four groups afterincubating mouse sympathetic neurons with serum IgG (P>0.05). However, NET mRNA expression levels in mouse sympathetic neurons were significantly higher after administration of serum IgG from the CP/CPPS+ED and CP/CPPS groups compared with the ED and control groups (P<0.05); the ED and control groups showed comparable values (P<0.05). Meanwhile, NET protein expression levels were significantly higher (P<0.05) in the CP/CPPS+ED group compared with the CP/CPPS, ED and control groups, which showed similar values (P>0.05). Serum IgG from LUTS-dominated CP/CPPS patients accompanied by ED affects NET expression in mouse sympathetic neurons.