斑点灯笼蝇卵寄生蜂东方小蠹的隐遗传多样性及相关生态差异

Yunke Wu, Hannah J. Broadley, Kendra A. Vieira, J. McCormack, Corrine A. Losch, Hyeban Namgung, Y. Kim, Hyojoong Kim, Alana R. McGraw, Marjorie Z. Palmeri, Seung-Hyeop Lee, L. Cao, Xiaoyi Wang, J. Gould
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引用次数: 3

摘要

东方小黄蜂(Anastatus orientalis)是斑点灯笼蝇(Lycorma delicatula)的卵寄生蜂,原产于中国北方,在美国作为一种潜在的生物防治剂正在进行试验。作为研究的一个组成部分,我们在中国北京和烟台两地采集了东方盲蝽,并在美国进行了密闭饲养。结果表明,东方盲蝽的滞育行为与其他研究者之前使用的饲养条件不同。为了了解导致重要生活史性状差异的主要机制,我们使用分子工具研究了来自中国和韩国的东方桦的遗传组成,这两个国家的东方桦已被引入以帮助入侵的L. delicatula的种群管理。线粒体DNA的分子分析恢复了六个单倍型组,它们在收集点之间表现出偏倚的丰度频率。一些单倍型分布广泛,而另一些单倍型只出现在特定的位置。在不同年份或羽化季节收集的黄蜂之间没有明显的模式。在控制群内变异后,单倍型群体间的未校正遗传距离为0.44% ~ 1.44%。东方桦遗传变异的特点是具有高度的局部多样性,而缺乏大尺度的种群结构。与本土人口相比,引进的韩国人口的遗传多样性较低。此外,我们还通过实验室饲养为主要单倍型群体建立了同雌系。滞育行为的差异与线粒体单倍型有关。结果表明,这些生活史特征具有一定的遗传基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cryptic genetic diversity and associated ecological differences of Anastatus orientalis, an egg parasitoid of the spotted lanternfly
Anastatus orientalis, native to northern China, is an egg parasitoid wasp of the spotted lanternfly (Lycorma delicatula) and is being tested as a potential biological control agent for invasive L. delicatula in the United States. As a component of these evaluations, live A. orientalis collected from Beijing and Yantai in China were reared in containment in the U.S. These specimens showed different responses in diapause behaviors to rearing conditions used previously by other researchers. To understand the primary mechanism potentially driving discrepancies in important life history traits, we used molecular tools to examine the genetic composition of A. orientalis from China and from South Korea, where the parasitoid has been introduced to aid in the population management of invasive L. delicatula. Molecular analysis of mitochondrial DNA recovered six haplotype groups, which exhibit biased frequency of abundance between collection sites. Some haplotypes are widespread, and others only occur in certain locations. No apparent pattern is observed between wasps collected from different years or emergence seasons. Uncorrected genetic distances between haplotype groups range from 0.44% to 1.44% after controlling for within-group variation. Genetic variance of A. orientalis is characterized by high levels of local diversity that contrasts with a lack of a broad-scale population structure. The introduced Korean population exhibits lower genetic diversity compared to native populations. Additionally, we created iso-female lines for major haplotype groups through laboratory rearing. Differences in diapause behavior were correlated with mitochondrial haplotype. Our results indicate that the observed life history traits in A. orientalis have a genetic base.
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