The Effect of He-Ne laser Radiation and Temperature of Blood in Vitro on the Erythrocytes Sedimentation Rate Values in Healthy People

Background : The aim of this study is to research the in vitro effect of the He-Ne laser radiation and study the effect of environmental temperature on Erythrocyte Sedimentation Rate (ESR) in blood samples from healthy . Objective : To show the effect of He-Ne laser and effect of environmental temperature on Erythrocyte Sedimentation Rate (ESR) Subject & Methods : He-Ne laser wave length of 632.8ηm was used for irradiation with 0.5mm diameter beam spot on blood samples, with power density 1mw/cm 2 , The irradiation times were (1hr ) , The samples of blood were obtained 30 contributors and each sample was separated into three samples for irradiation and control . Results : The results showed that there were significant differences in the values of sedimentation rates for both class (25 -45 ) 0 c degree heat compared with the values of sedimentation 25 0 c degree heat temperature for irradiation and control . It shows the effect of temperature factor on the red blood cells sedimentation rate in blood samples from healthy people and see the effect of the degree of laboratory heat due to lack of efficient heating and cooling devices in the summer and winter on the sedimentation rates of values to be taken into consideration with the results . Conclusion : The change of temperature (high and low) has a great effect on the deposition and precipitation of red blood cells in health people and thus must be taken into consideration and this effect is also observed with the use of He - Ne laser at a temperature of 25 ° C.