鱼类养殖食品中沙门氏菌的免疫学和生物传感检测技术综述

Jerônimo Vieira Dantas Filho, Douglas Guedes Gotardi, J. Cavali, Sandro de Vargas Schons
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引用次数: 0

摘要

沙门氏菌病是世界上最常见的食源性疾病。在巴西,受沙门氏菌污染的食品在统计数据中名列前茅。因此,本研究的目的是通过生物技术知识,编制检测食品中沙门氏菌的替代和创新技术,如养鱼衍生物、免疫学和生物传感技术。这是一个定性的描述性探索性数据调查,旨在数据分析。研究和数据收集是在书目数据库中进行的:学术谷歌、Scielo、CAPES期刊和机构数据库,使用葡萄牙语和英语的特定描述符,单词和术语用布尔运算符“and”和“OR”分隔。有一些创新的替代方法可用于识别食物中是否存在沙门氏菌。尽管免疫学和生物感觉技术在科学文献中不如分子方法常见,但它们是具有高特异性和敏感性的技术。这些技术是近年来在鱼类中最发达的替代方法。而且,他们可以在生物识别中使用分子和免疫学技术,其特点是不需要对样品进行预富集。根据文献发现,本研究所涉及的技术具有快速响应的特点,加快了研究人员和技术人员的决策速度,具有很好的工业应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IMMUNOLOGICAL AND BIOSSENSORY TECHNIQUES FOR DETECTION OF SALMONELLA IN FOOD DERIVED FROM FISH FARMING - REVIEW
Salmonellosis is the world's most common foodborne illness. In Brazil, foods contaminated by salmonella lead the statistics. Therefore, the aim of this study is, through biotechnological knowledge, to compile alternative and innovative techniques for the detection of salmonella in foods, such as fish-farming derivatives, immunological and biosensorial techniques. This is a descriptive exploratory data survey of a qualitative nature, aiming at data analysis. Research and data collection were carried out in bibliographic databases: Academic Google, Scielo, CAPES journals and institutional repositories using specific descriptors - in Portuguese and English, with words and terms separated by the Boolean operators 'AND' and 'OR'. Some innovative and alternative methods are available to identify the presence of salmonella in food. Immunological and biosensory techniques, despite being less frequent in the scientific literature than molecular methods, are techniques that present high specificity and sensitivity. These techniques have been the most developed alternative methods in fish in recent years. And, they can employ both molecular and immunological techniques in biorecognition, which is characterized as an advantage of not having a requirement for pre-enrichment of the sample. According to the literature found, the techniques covered in this study are quick to respond, which speeds up decision-making by researchers and technicians, which makes the techniques very promising for industrial application.
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