V Stocchi, L Masat, B Biagiarelli, A Accorsi, G Piccoli, F Palma, L Cucchiarini, M Dachà
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High resolution of multiple forms of red blood cell enzymes using a Toyopearl DEAE 650 S.
We have investigated a new anion exchange chromatographic support (Toyopearl DEAE 650 S) which simultaneously allows easily to remove hemoglobin from hemolysates and to obtain a very high resolution of enzymes present in multiple forms. The results obtained are better than those obtainable using an anion-exchange HPLC column. The data obtained at analytical level suggest a wider use of this new matrix also for preparative purposes without significant changes in the resolution.
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