Bioremediation of crude oil spillage using Cisuss populnea leave as bio-sorbent

The total petroleum hydrocarbon concentration decrease at the initial and final bioremediation across different variable of cisuss populnea leave biosorbent. 25 g of soil was weighed into different plate, contaminated with about 10 ml of crude oil, the plate was labeled as BI, CI DI, BF, CF and DF (leave) across 3 g, 6 g, and 9 g variables and A (soil and crude oil mixture) as control for initial and final bioremediation. 1 g of each contaminated soil sample was extracted using mechanical shaker at room temperature and the extracts were analyzed using GC/MS the results showed that the initial treatment of leave biosorbent were 706.4482 ppm, 544.2509 ppm, 347.75909 ppm respectively. After 4 weeks of biodegradation, the concentration decreases to 310.4318 ppm, 117.8105 ppm, 60.05455pmm and control 757.39864 ppm respectively. The Total removal efficiencies of Total petroleum hydrocarbon were 56.2%, 78.4%, and 82.7% for the treatments of 3 g, 6 g, and 9 g of leaves sorbent