A. Najafi , M.M. Alauddin , A. Sosa , G.Q. Ma , D.C.P. Chen , A.L. Epstein , M.E. Siegel
{"title":"用N2S4螯合物体外和体内对荷瘤裸鼠进行186re标记抗体的评价","authors":"A. Najafi , M.M. Alauddin , A. Sosa , G.Q. Ma , D.C.P. Chen , A.L. Epstein , M.E. Siegel","doi":"10.1016/0883-2897(92)90009-N","DOIUrl":null,"url":null,"abstract":"<div><p>We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N<sub>2</sub>S<sub>3</sub>. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with <sup>186</sup>Re and their biological integrity was evaluated <em>in vitro</em> and <em>in vivo</em>. <sup>186</sup>Re-labeled antibodies using N<sub>2</sub>S<sub>4</sub> methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi <sup>186</sup>Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.</p></div>","PeriodicalId":14328,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","volume":"19 2","pages":"Pages 205-212"},"PeriodicalIF":0.0000,"publicationDate":"1992-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0883-2897(92)90009-N","citationCount":"18","resultStr":"{\"title\":\"The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice\",\"authors\":\"A. Najafi , M.M. Alauddin , A. Sosa , G.Q. Ma , D.C.P. Chen , A.L. Epstein , M.E. Siegel\",\"doi\":\"10.1016/0883-2897(92)90009-N\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N<sub>2</sub>S<sub>3</sub>. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with <sup>186</sup>Re and their biological integrity was evaluated <em>in vitro</em> and <em>in vivo</em>. <sup>186</sup>Re-labeled antibodies using N<sub>2</sub>S<sub>4</sub> methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi <sup>186</sup>Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.</p></div>\",\"PeriodicalId\":14328,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"volume\":\"19 2\",\"pages\":\"Pages 205-212\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0883-2897(92)90009-N\",\"citationCount\":\"18\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088328979290009N\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088328979290009N","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The evaluation of 186Re-labeled antibodies using N2S4 chelate in vitro and in vivo using tumor-bearing nude mice
We have recently described a method for radiolabeling monoclonal antibodies, with metallic radionuclides using a new chelating agent N2S3. Using this chelate the monoclonal antibodies Lym-1 and B72.3 were labeled with 186Re and their biological integrity was evaluated in vitro and in vivo. 186Re-labeled antibodies using N2S4 methodology were found to be stable in human serum and retained their immunoreactivity. Intravenous administration of 0.5 mCi 186Re-labeled antibodies resulted in partial or complete regression of tumor tissue in mice.
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