应用计算机RFLP方法对石竹属植物MC1R基因等位基因进行了研究

Sustainable use and protection of animal world in the context of climate change Pub Date : 2021-09-01 DOI:10.53937/icz10.2021.61

Victor Sîtnic

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引用次数: 0

摘要

摘要在本研究中，我们旨在使用生物信息学算法来预测猪MC1R等位基因的RFLP片段，模拟超过700种限制性内切酶的序列消化。结果表明，几种限制性内切酶有潜力用于苏斯scrofa个体的基因分型以及杂种和纯种野猪/家猪(DP)的区分。对摩尔多瓦种群中的野猪和家猪进行基因分型研究将有助于确定遗传渗入/回交程度，并有助于制定适当的保护措施

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Using in silico RFLP method for the study of MC1R gene alleles in the species Sus Scrofa

Abstract. In the present study we aimed to use a bioinformatics algorithm that predict RFLP fragmentation of swine MC1R alleles simulating the sequence digestion with over 700 restriction enzymes. The results show several restriction enzymes that have the potential to be used for genotyping of Sus scrofa individuals and for differentiation between hybrids and pure line wild boars (WB)/domestic pigs (DP). Genotyping studies of wild boars and domestic pigs in Moldova populations would allow the determination of introgression/backcrossing degree and would contribute to the elaboration of adequate conservation measures

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Sustainable use and protection of animal world in the context of climate change

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