独特型网络对小鼠免疫球蛋白G3抗碳水化合物抗体的反应。

D R Anderson, R E McCoobery
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引用次数: 1

摘要

制备了两种小鼠单克隆抗体8A6和8C2,以对抗肿瘤相关二联胞脂苷的碳水化合物部分。两种抗体均为免疫球蛋白G3 (IgG3) K亚型亚类。其中一种抗体(8A6)作为网络免疫原用于产生抗独特型抗体。鉴定出2种AB2抗独特型抗体。一个AB2 (12E5)随后被证明识别8A6 kappa轻链的线性表位。第二个AB2 (9H8)识别依赖于独特型三级结构维持的构象表位。将这两种抗独特型分别注射到(同基因)小鼠和(异种)家兔体内,以评估它们作为“网络”抗原促进AB3和抗碳水化合物AB1反应的有效性。来自同基因和异种宿主的AB3群体被发现具有独特型特异性,但无法产生可测量的抗肿瘤亚群(AB1')。这些研究表明,IgG3同型可能不是碳水化合物表位模仿的合适的独特型模板。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Idiotype network responses to murine immunoglobulin G3 anti-carbohydrate antibodies.

Two murine monoclonal antibodies, 8A6 and 8C2, were generated against the carbohydrate moiety of tumor-associated disialoganglioside. Both of the antibodies were of the immunoglobulin G3 (IgG3), K isotype subclass. One of these antibodies (8A6) was used as a network immunogen for the generation of anti-idiotype antibodies. Two AB2 anti-idiotype antibodies were identified. One AB2 (12E5) was subsequently shown to recognize a linear epitope of the 8A6 kappa light chain. The second AB2 (9H8), recognizes a conformational epitope which is dependent on the maintenance of the tertiary structure of the idiotype. Both anti-idiotypes were injected into (syngeneic) mice and (xenogeneic) rabbits to evaluate their effectiveness as "network" antigens in promoting AB3 and anti-carbohydrate AB1' responses. AB3 populations from both syngeneic and xenogeneic hosts were found to be idiotype-specific, yet were unable to produce a measurable subpopulation of anti-tumor (AB1'). These studies suggest that IgG3 isotypes may not be suitable idiotype templates for the mimicry of carbohydrate epitopes.

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