电动和克尔效应对琼脂糖电泳凝胶的电双折射图像的贡献。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1992-01-01

M Lanan, M D Morris

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引用次数: 0

摘要

多线性回归分析用于测试琼脂糖电泳凝胶中DNA片段带的电双折射成像（EBI）的模型（Lanan等人，1991）。通过将溴化乙锭荧光谱与EBI结果拟合，研究了归因于局部电动凝胶畸变和固有DNA双折射的EBI信号。荧光偏振成像（FPI）用于评估局部凝胶畸变对片段带上核酸取向的影响。研究表明，DNA与施加的电场平均平行排列，与其在带内的位置无关。在所测试的条件下，DNA取向随着电场强度的1.2次方而变化。

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Electrokinetic and Kerr effect contributions to electric birefringence images of agarose electrophoresis gels.

Multilinear regression analysis is used to test a model (Lanan et al., 1991) for electric birefringence imaging (EBI) of DNA fragment bands in agarose electrophoresis gels. EBI signals attributed to localized electro-kinetic gel distortion and to intrinsic DNA birefringence are studied by fitting ethidium bromide fluorescence profiles to EBI results. Fluorescence polarization imaging (FPI) is used to assess the influence of localized gel distortion on nucleic acid orientation across a fragment band. It is shown that DNA aligns parallel, on average, with an applied electric field independent of its location within a band. DNA orientation varies as the 1.2 power in electric field strength under the conditions tested.

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Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society

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