{"title":"α-糖蛋白和β-环糊精固定相对(RS)‐普萘洛尔外消旋体的比较手性分离","authors":"Derouicha Matmour","doi":"10.57056/ajb.v2i2.47","DOIUrl":null,"url":null,"abstract":"This paper describes a comparative study of tow chiral separations of (RS)‐Propranolol racemate by HPLC using α-Glycoprotein (AGP) and β-Cyclodextrin (BCD) Stationary Phases. For the AGP separation, the column size was (150 mm X4 mm X 5 μm), the mobile phase composed of Propanol-2 and Ammonium acetate (0.5:99.5 v/v), at a flow rate of 0.9 mL/min and the detection by ultraviolet absorption at 225 nm. For the BCD separation, the column size was (250 mm X4 mm X 5 μm), the mobile phase composed of Acetonitrile: Ethanol: Acetic acid: Triethylamine (960: 40: 4: 3 v/v/v/v), at a flow rate of 1 mL/min and the detection by ultraviolet absorption at 225 nm. The retention time of S-Propranolol and R-Propranolol with AGP separation was respectively: 7.25 min and 11.82 min while with the BCD separation 16.18 min and 18.50 min respectively. The racemate contains 50.46 % of S-Propranolol and 49.53 % of R-Propranolol with AGP separation while with BCD separation, it contains 50.43 %/49.57 %. There is a similarity between the enantiomeric purity values and the enantiomeric excess values of tow separations, but the separation with AGP stationary phase is faster than with the BCD stationary phase. For a selective β-blocking use, it could be very interesting to encourage its production in its form enantiomerically pure wich is the S-enantiomer.","PeriodicalId":431221,"journal":{"name":"Algerian Journal of Biosciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative Chiral Separation of (RS)‐Propranolol Racemate by HPLC Using α-Glycoprotein and β-Cyclodextrin Stationary Phases.\",\"authors\":\"Derouicha Matmour\",\"doi\":\"10.57056/ajb.v2i2.47\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This paper describes a comparative study of tow chiral separations of (RS)‐Propranolol racemate by HPLC using α-Glycoprotein (AGP) and β-Cyclodextrin (BCD) Stationary Phases. For the AGP separation, the column size was (150 mm X4 mm X 5 μm), the mobile phase composed of Propanol-2 and Ammonium acetate (0.5:99.5 v/v), at a flow rate of 0.9 mL/min and the detection by ultraviolet absorption at 225 nm. For the BCD separation, the column size was (250 mm X4 mm X 5 μm), the mobile phase composed of Acetonitrile: Ethanol: Acetic acid: Triethylamine (960: 40: 4: 3 v/v/v/v), at a flow rate of 1 mL/min and the detection by ultraviolet absorption at 225 nm. The retention time of S-Propranolol and R-Propranolol with AGP separation was respectively: 7.25 min and 11.82 min while with the BCD separation 16.18 min and 18.50 min respectively. The racemate contains 50.46 % of S-Propranolol and 49.53 % of R-Propranolol with AGP separation while with BCD separation, it contains 50.43 %/49.57 %. There is a similarity between the enantiomeric purity values and the enantiomeric excess values of tow separations, but the separation with AGP stationary phase is faster than with the BCD stationary phase. For a selective β-blocking use, it could be very interesting to encourage its production in its form enantiomerically pure wich is the S-enantiomer.\",\"PeriodicalId\":431221,\"journal\":{\"name\":\"Algerian Journal of Biosciences\",\"volume\":\"15 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Algerian Journal of Biosciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.57056/ajb.v2i2.47\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Algerian Journal of Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.57056/ajb.v2i2.47","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用α-糖蛋白(AGP)和β-环糊精(BCD)固定相对(RS)‐外消旋普萘洛尔进行了两种手性分离研究。AGP的分离柱尺寸为(150 mm X4 mm X 5 μm),流动相为丙醇-2和乙酸铵(0.5:99.5 v/v),流速为0.9 mL/min,紫外吸收检测波长为225 nm。色谱柱尺寸为(250 mm X4 mm X 5 μm),流动相为乙腈:乙醇:乙酸:三乙胺(960:40:4:3 v/v/v/v),流速为1 mL/min,紫外吸收检测波长为225 nm。AGP分离s -普萘洛尔和r -普萘洛尔的保留时间分别为7.25 min和11.82 min, BCD分离s -普萘洛尔的保留时间分别为16.18 min和18.50 min。AGP分离时,s -心得安的含量为50.46%,r -心得安的含量为49.53%;BCD分离时,s -心得安的含量为50.43%,r -心得安的含量为49.57%。两种分离方法的对映体纯度值和对映体过量值相似,但AGP固定相的分离速度比BCD固定相快。对于选择性β阻断的用途,鼓励其以对映体纯形式(即s -对映体)生产可能是非常有趣的。
Comparative Chiral Separation of (RS)‐Propranolol Racemate by HPLC Using α-Glycoprotein and β-Cyclodextrin Stationary Phases.
This paper describes a comparative study of tow chiral separations of (RS)‐Propranolol racemate by HPLC using α-Glycoprotein (AGP) and β-Cyclodextrin (BCD) Stationary Phases. For the AGP separation, the column size was (150 mm X4 mm X 5 μm), the mobile phase composed of Propanol-2 and Ammonium acetate (0.5:99.5 v/v), at a flow rate of 0.9 mL/min and the detection by ultraviolet absorption at 225 nm. For the BCD separation, the column size was (250 mm X4 mm X 5 μm), the mobile phase composed of Acetonitrile: Ethanol: Acetic acid: Triethylamine (960: 40: 4: 3 v/v/v/v), at a flow rate of 1 mL/min and the detection by ultraviolet absorption at 225 nm. The retention time of S-Propranolol and R-Propranolol with AGP separation was respectively: 7.25 min and 11.82 min while with the BCD separation 16.18 min and 18.50 min respectively. The racemate contains 50.46 % of S-Propranolol and 49.53 % of R-Propranolol with AGP separation while with BCD separation, it contains 50.43 %/49.57 %. There is a similarity between the enantiomeric purity values and the enantiomeric excess values of tow separations, but the separation with AGP stationary phase is faster than with the BCD stationary phase. For a selective β-blocking use, it could be very interesting to encourage its production in its form enantiomerically pure wich is the S-enantiomer.