{"title":"共聚焦荧光显微镜的光学层析成像","authors":"O. Nakamura, S. Kawata, S. Minami","doi":"10.1364/srs.1989.fd1","DOIUrl":null,"url":null,"abstract":"In the past few years, microscope tomography has been intensively studied.1-5 Agard and Sedat restored the 3-D structure of biological cells from the focus series images given by a conventional epi-fluorescence microscope, by inverting the 3-D optical transfer function of the system.1 However, since the 3-D OTF of an epi-fluorescence microscope is angularly band-limited,6 the 3-D spatial frequency components only within the angular band can be restored in their method. As a result, the longitudinal resolution in the restored 3-D structure is not at all satisfactory.","PeriodicalId":193110,"journal":{"name":"Signal Recovery and Synthesis III","volume":"27 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Optical Tomography by Confocal Fluorescence Microscope\",\"authors\":\"O. Nakamura, S. Kawata, S. Minami\",\"doi\":\"10.1364/srs.1989.fd1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the past few years, microscope tomography has been intensively studied.1-5 Agard and Sedat restored the 3-D structure of biological cells from the focus series images given by a conventional epi-fluorescence microscope, by inverting the 3-D optical transfer function of the system.1 However, since the 3-D OTF of an epi-fluorescence microscope is angularly band-limited,6 the 3-D spatial frequency components only within the angular band can be restored in their method. As a result, the longitudinal resolution in the restored 3-D structure is not at all satisfactory.\",\"PeriodicalId\":193110,\"journal\":{\"name\":\"Signal Recovery and Synthesis III\",\"volume\":\"27 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Signal Recovery and Synthesis III\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1364/srs.1989.fd1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Signal Recovery and Synthesis III","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1364/srs.1989.fd1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Optical Tomography by Confocal Fluorescence Microscope
In the past few years, microscope tomography has been intensively studied.1-5 Agard and Sedat restored the 3-D structure of biological cells from the focus series images given by a conventional epi-fluorescence microscope, by inverting the 3-D optical transfer function of the system.1 However, since the 3-D OTF of an epi-fluorescence microscope is angularly band-limited,6 the 3-D spatial frequency components only within the angular band can be restored in their method. As a result, the longitudinal resolution in the restored 3-D structure is not at all satisfactory.
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