A Review of Artificial Genetic Constructs and Their Applications as Positive Controls

Advances in nucleic acid based molecular diagnosis techniques, provided the basis for efficient, accurate and rapid detection of bi-ological/pathological agents. However, these techniques suffer from major limitations including lack of the genomic sequence of high-riskpathogenstocalibratetechniquesaspositivecontrols,falsepositiveresultsandrestrictionsonaccesstoexistingcommer-cial tests. However with the advent of synthetic biology, it is possible to construct appropriate positive controls. Artificial genetic constructsarethesyntheticconstructsthatcontaingeneticmaterialsfromhighlydangerousbacteriaorviruses,whichcanbeused as positive controls in molecular techniques such as PCR. In this review, we introduce positive controls and simulator positive controls firstly and discuss about artificial genetic constructs, procedure of design and synthesis secondly. Finally, we discussed about single and chimeric artificial genetic constructs as two main categories of these positive control. introduce positive controls and simulator positive controls; then, we discuss about artificial genetic constructs as well as approaches for their design and synthesis of them. Finally, we provide an overview of two main categories of the simulator pos-itives, including single and chimeric synthetic artificial constructs.