Formulasi Inokulan Biosintetik dan Pembinaan Model Statistik Verhulst

Penyelidikan yang dilakukan bertujuan mensintesis, mengformulasi dan menilai inokulan biosintetik bagi penghasilan resin gaharu. Bagi mengformulasi inokulan biosintetik, beberapa bahan digunakan termasuklah madu lebah, susu segar, sukros, air kelapa, dan kulat Fusarium solani yang diadun dengan menggunakan sukatan tertentu. Inokulan biosintetik diformulasi di makmal Biologi, Fakulti Sains dan Matematik, UPSI. Inokulan biosintetik yang diformulasi diuji terhadap tujuh batang pokok gaharu di Slim River, Perak dengan teknik suntikan lubang bor, suntikan titisan mata, dan sapuan. Hasil resin gaharu dituai dalam tempoh enam bulan dari tarikh suntikan. Sampel resin gaharu dipungut diuji di makmal Kimia, FSM, UPSI. Kualiti resin gaharu dianalisis dengan teknik Kromatografi Gas-Spektrometri Jisim (GCMS). Parameter kualiti resin gaharu yang diamati adalah warna dan keharuman. Manakala populasi Fusarium solani dalam inokulan biosintetik dianggar dengan menggunakan model statistik Verhulst. Dapatan penyelidikan menunjukkan bahawa inokulan biosintetik yang diformulasi berjaya menghasilkan resin gaharu dalam jangka masa enam bulan dengan teknik suntikan lubang bor dan titisan mata. Model statistik Verhulst populasi Fusarium solani pada masa (t) yang dibina adalah x(t)=k/(1+(k/x_0 - 1)e^ -rt) . Kesimpulannya, penyelidikan ini berjaya mengformulasi inokulan biosintetik dan membina model statistik Verhulst bagi menganggar populasi Fusarium solani. Implikasi daripada penyelidikan ini adalah inokulan biosintetik yang terformulasi sesuai digunakan dalam penghasilan resin gaharu dan boleh dikomersialkan. ----------------------------------------------------------------- The research aimed at synthesizing, formulating and evaluating biosynthetic inoculant in producing of agarwood resins. In order to formulate biosynthetic inoculant, some of the ingredients used include bee honey, fresh milk, sucrose, coconut water, and Fusarium solanium fungus which were mixed at a certain measurement level. The biosynthetic inoculant was formulated in Biology laboratory, Faculty of Science and Mathematics, UPSI. The formulated inoculant was tested on seven agarwood trees in Slim River, Perak using hole drilling, eye drop injection techniques, and wiping. The agarwood resin was harvested six months after the injection process. The sample of agarwood resin was tested in Chemistry laboratory, Faculty of Science and Mathematics, UPSI. The quality of the agarwood resin was analyzed using the Gas Chromatography-Mass Spectrometry (GCMS) technique. The quality parameters of the agarwood resin that were observed were the color and fragrance. The Fusarium solani population of biosynthetic inoculant was estimated using Verhulst’s statistical model. The research findings revealed that the formulated biosynthetic inoculation successfully produced agarwood resin within a period of six months with hole drilling and eye drops injection techniques. The Verhult’s statistical model of the Fusarium solani population at time (t) was developed as x(t)=k/(1+(k/x_0 - 1)e^ -rt). In conclusion, the research was successful in formulating biosynthetic inoculant and developed Verhulst’s statistical model in estimating Fusarium solani population. The implications of this research were that the formulated biosynthetic inoculant is apposite in producing agarwood resin and therefore can be commercialized. Keywords: Biosynthetic inoculant, Verhult’s statistical model